Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam.

Aquilaria species (Thymelaeaceae family) are an important botanical resources for the fragrant agarwood. Agarwood is mainly composed of terpenoids that are produced by a family of enzyme called terpene synthases (TPS). The species have been studied for the decades, however, the size of its gen...

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Main Author: Azman, Farah Hanani
Format: Thesis
Language:English
Published: 2018
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Online Access:http://psasir.upm.edu.my/id/eprint/84450/1/FH%202019%2012%20-%20ir.pdf
http://psasir.upm.edu.my/id/eprint/84450/
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.844502022-01-03T08:05:32Z http://psasir.upm.edu.my/id/eprint/84450/ Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam. Azman, Farah Hanani Aquilaria species (Thymelaeaceae family) are an important botanical resources for the fragrant agarwood. Agarwood is mainly composed of terpenoids that are produced by a family of enzyme called terpene synthases (TPS). The species have been studied for the decades, however, the size of its genome which are important for breeding effort is yet to be analyzed. In addition, identification of terpenoid biosynthesis gene in Aquilaria spp. is critical for a deeper understanding of the mechanisms involved in agarwood formation. In this study, the genome size of five Aquilaria species which include A. hirta, A. malaccensis, A. subintegra, A. sinensis, and A. microcarpa. were estimated by using flow cytometry. The results showed no large variation in the 2C- values of the five species (1.35 to 2.23 pg). The TPS genes was analyzed in one the five species, A. malaccensis based on the previously developed in-house transriptome database. Putative TPS transcripts in A. malaccensis encoding monoterpene, sesquiterpene and diterpene were found clustered within known TPS families. A transcript encoding linalool synthase, an acyclic monoterpene common to floral scents of numerous plant was selected for protein expression analysis in E. coli where the protein accumulated in insoluble form. Collectively, the information obtained on the 2C-values can help elucidate the underlying mechanism in the evolutionary forces driving genome size variation in Aquilaria species while identification of putative TPS transcripts in A. malaccensis provides a clue for the role of TPS in agarwood formation in that particular species. 2018-11 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/84450/1/FH%202019%2012%20-%20ir.pdf Azman, Farah Hanani (2018) Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam. Masters thesis, Universiti Putra Malaysia. Aquilaria malaccensis Terpenes Agarwood (Wood)
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Aquilaria malaccensis
Terpenes
Agarwood (Wood)
spellingShingle Aquilaria malaccensis
Terpenes
Agarwood (Wood)
Azman, Farah Hanani
Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam.
description Aquilaria species (Thymelaeaceae family) are an important botanical resources for the fragrant agarwood. Agarwood is mainly composed of terpenoids that are produced by a family of enzyme called terpene synthases (TPS). The species have been studied for the decades, however, the size of its genome which are important for breeding effort is yet to be analyzed. In addition, identification of terpenoid biosynthesis gene in Aquilaria spp. is critical for a deeper understanding of the mechanisms involved in agarwood formation. In this study, the genome size of five Aquilaria species which include A. hirta, A. malaccensis, A. subintegra, A. sinensis, and A. microcarpa. were estimated by using flow cytometry. The results showed no large variation in the 2C- values of the five species (1.35 to 2.23 pg). The TPS genes was analyzed in one the five species, A. malaccensis based on the previously developed in-house transriptome database. Putative TPS transcripts in A. malaccensis encoding monoterpene, sesquiterpene and diterpene were found clustered within known TPS families. A transcript encoding linalool synthase, an acyclic monoterpene common to floral scents of numerous plant was selected for protein expression analysis in E. coli where the protein accumulated in insoluble form. Collectively, the information obtained on the 2C-values can help elucidate the underlying mechanism in the evolutionary forces driving genome size variation in Aquilaria species while identification of putative TPS transcripts in A. malaccensis provides a clue for the role of TPS in agarwood formation in that particular species.
format Thesis
author Azman, Farah Hanani
author_facet Azman, Farah Hanani
author_sort Azman, Farah Hanani
title Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam.
title_short Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam.
title_full Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam.
title_fullStr Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam.
title_full_unstemmed Analysis of genome size of Aquilaria species and characterization of terpene synthase transcripts of Aquilaria malaccensis Lam.
title_sort analysis of genome size of aquilaria species and characterization of terpene synthase transcripts of aquilaria malaccensis lam.
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/84450/1/FH%202019%2012%20-%20ir.pdf
http://psasir.upm.edu.my/id/eprint/84450/
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