Isolation and analysis of rhlC and rhlR from rhamnolipid producing bacterium, Pseudomonas aeruginosa strain S5
Rhamnolipids are biomolecules with a huge potential in leading global surfactant market, which find applications in various kind of industry in demand for emulsifying and solubilizing agent including food industry, pharmaceutical industry, and petroleum ind...
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| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/85051/1/FBSB%202015%20117%20-%20IR.pdf http://psasir.upm.edu.my/id/eprint/85051/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | Rhamnolipids are biomolecules with a huge potential in leading global surfactant
market, which find applications in various kind of industry in demand for
emulsifying and solubilizing agent including food industry, pharmaceutical industry, and
petroleum industry. Rhamnolipids are being majorly produced by microorganism,
particularly, Pseudomonas aeruginosa. This study aims to test rhamnolipid production in
Pseudomonas aeruginosa strain S5 and to analyze the genes involve for the rhamnolipids
biosynthesis which are rhlC and rhlR that code for rhamnosyltransferase 2 and transcription
regulator respectively. Qualitative test on cetyltrimethyl ammonium bromide agar and
quantitative tests by emulsification index valued 37.44 %, display capabilities of Pseudomonas
aeruginosa strain S5 in producing rhamnolipid. In molecular detection of rhamnolipid
production, BLAST result shows high similarity between both rhlC and rhlR sequences, and
sequence available in database. The information from the gene analysis could facilitate
the understanding on the rhamnolipid biosynthesis in Pseudomonas aeruginosa strain S5,
hence can be further used in genetic modulation in order to meet future
prospects. |
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