Isolation and characterization of bacteriophage from crab

• Main Author: Mohamad Safiee, Amira Wahida
• Format: Project Paper Report
• Language: English
• Published: 2015
• Online Access: http://psasir.upm.edu.my/id/eprint/85104/1/FBSB%202015%2093%20-%20IR.pdf; http://psasir.upm.edu.my/id/eprint/85104/
• Institution: Universiti Putra Malaysia

This project was carried out to isolate, purify and characterize the bacteriophage from the crab (Callinectus sapidus) also known as blue crab. The crab was obtained freshly from Jetty Wak Sempo, Bagan Lalang, Sepang. Seven bacteria was isolated from the crab and labelled as A, B, C, D, E, F and G were used as a screening agent for the isolation of the bacteriophage. Besides, another 5 species of known bacteria were used for bacteriophage isolation. The bacteria were Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus and Staphylococcus aureus. All 12 species of bacteria were used but only bacteria C was infected by the bacteriophage. The bacteriophage was labelled as C-CC. The bacteriophage infected bacteria was identified by gram staining and biochemical tests. Based on the gram staining, the bacterium is a gram negative bacterium with coccobacillus shape. Then, the isolated bacteriophage was characterized based on their protein, genomic, biology and physical characteristics. At the end of the study, the isolated bacteriophage was confirmed classified as DNA-containing bacteriophage with lytic life cycle. The bacteriophage could be as used in the phage therapy against the bacterial contamination.