Breast cancer aggressiveness, miRNA and choline

• Main Authors: Shaharuddin, Shazreen, Ahmad Saad, Fathinul Fikri, Osman, Muhamed T.
• Format: Book
• Language: English; English
• Published: Independently Published 2020
• Online Access: http://psasir.upm.edu.my/id/eprint/85773/1/Breast%20Cancer%20Aggressiveness%2C%20miRNA%20and%20Choline.jpg; http://psasir.upm.edu.my/id/eprint/85773/7/Breast%20cancer%20aggressiveness%2C%20miRNA%20and%20choline.pdf; http://psasir.upm.edu.my/id/eprint/85773/; https://www.amazon.com/Breast-Cancer-Aggressiveness-miRNA-Choline/dp/B085DRDXK2
• Institution: Universiti Putra Malaysia

The level of choline kinase in human breast cancer cells was found to be overexpress as compared to normal human mammary epithelial cells. This book presents a study that we investigated the potential expression of choline in 18F-FCH PET/CT as compared to F18Fluorodeoxyglucose PET/CT and correlations with expression of miRNA, CD47 and histo-immunochemical markers of estrogen, progesterone and HER2 in detecting aggressiveness of breast cancer.Twenty one patients with Birads 4 or 5 on mammogram and recurrent breast carcinoma underwent imaging of 18F-Fluorocholine and 18F-Fluorodeoxyglucose PET/CT. Tissue biopsy and histo-immunochemical results with blood sampling of expression of miRNA-21,miRNA-155 and CD47 were recorded. The data were evaluated by experienced nuclear imaging using the biopsy and histo-immunochemical findings as a gold standard. There were 21 females with a mean age of 52.82 ± 10.71 years. There is a significant evidence of high uptake of 18F-Fluorocholine PET/CT in the metastatic lesions (2.27±3.19) as compare to 18F-Fluorodeoxyglucose (1.74±2.32, p=0.004). While there is a significant association between high 18F-Fluorocholine uptake (3.53±3.51,p=0.005) with HER2-ve (1.98±2.14, p=0.009) in metastatic lesions and lymph node. High uptake of 18F-Fluorocholine showed a significant correlation with expression of miRNA-21 in lymph node (1.81±2.21,p=0.05) and metastasis lesions (3.33±3.61,p=0.02). There is also a significant correlation between high choline uptake with expression of miRNA-155 (1.47±1.99,p=0.01) and CD47 (0.85±0.23,p=0.008) in lymph node and 18F-Fluorocholine showed high sensitivity and specificity (40%,68.8%) compared to 18F-Fluorodeoxyglucose (27.3%,60%). There is a significant association miRNA-155 between patients with recurrent breast cancer and non-recurrent breast cancer (p=0.026) and expression of HER2 –ve (p=0.042).We concluded that higher choline uptake in 18F-Fluorocholine would potentially be used as novel surrogate marker for detection and prediction of an aggressive breast cancer with strong correlation with the protein overexpression of miRNA-155, miRNA-21,CD47 and HER2-ve.