Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
Despite pulmonary cryptococcosis showing good response to antifungal treatment, making accurate diagnosis in a timely manner remains a challenge. Tissue for fungal culture is less sensitive, nonspecific, and time-consuming to allow for prompt diagnosis. We herein report a case of 31-year-old woman...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fakulti Perubatan dan Sains Kesihatan, Universiti Putra Malaysia
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86813/1/Molecular%20Detection%20of%20Cryptococcus%20from%20Formalin.pdf http://psasir.upm.edu.my/id/eprint/86813/ https://medic.upm.edu.my/upload/dokumen/2020091012202011_2019_0210.pdf |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | Despite pulmonary cryptococcosis showing good response to antifungal treatment, making accurate diagnosis in
a timely manner remains a challenge. Tissue for fungal culture is less sensitive, nonspecific, and time-consuming
to allow for prompt diagnosis. We herein report a case of 31-year-old woman with no known medical illness but
presented with history of cough and haemoptysis for two months. The chest X-ray and bronchoscopy revealed
left lung mass. While tuberculosis showed negative result and no growth observed in the tissue culture, the
histopathological examination (HPE) finding was suggestive of fungal infection. The formalin-fixed
paraffin-embedded (FFPE) tissue was sent for molecular testing, which revealed Cryptococcus neoformans.
This report emphasises on the advantages and limitations of polymerase chain reaction (PCR) as an alternative
method to confirm the diagnosis in cases of culture-negative fungal infection. |
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