Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae

• Main Authors: Ishak, Siti Nor Hasmah, Ahmad Kamarudin, Nor Hafizah, Mohamad Ali, Mohd Shukuri, Leow, Adam Thean Chor, Mohd Shariff, Fairolniza, Raja Abd Rahman, Raja Noor Zaliha
• Format: Article
• Published: Public Library of Science 2021
• Online Access: http://psasir.upm.edu.my/id/eprint/95189/; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251751
• Institution: Universiti Putra Malaysia

5M mutant lipase was derived through cumulative mutagenesis of amino acid residues (D43E/T118N/E226D/E250L/N304E) of T1 lipase from Geobacillus zalihae. A previous study revealed that cumulative mutations in 5M mutant lipase resulted in decreased thermostability compared to wild-type T1 lipase. Mult...