Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum

Seven Bacillus spp. isolated from the marine water and the rhizosphere of the medicinal plant Coscinium fenestratum were studied to produce plant growth promotion (PGP) traits invitro. Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based on the 16S rRNA sequence,...

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Main Authors: Manasa, Mansani, Ravinder, Polapally, Gopalakrishnan, Subramaniam, Srinivas, Vadlamudi, Sayyed, R. Z., El Enshasy, Hesham Ali, Yahayu, Maizatulakmal, Tan, Ali Kee Zuan, Kassem, Hazem S., Hameeda, Bee
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Published: Multidisciplinary Digital Publishing Institute 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96429/
https://www.mdpi.com/2071-1050/13/21/12091
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.964292023-01-26T05:25:52Z http://psasir.upm.edu.my/id/eprint/96429/ Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum Manasa, Mansani Ravinder, Polapally Gopalakrishnan, Subramaniam Srinivas, Vadlamudi Sayyed, R. Z. El Enshasy, Hesham Ali Yahayu, Maizatulakmal Tan, Ali Kee Zuan Kassem, Hazem S. Hameeda, Bee Seven Bacillus spp. isolated from the marine water and the rhizosphere of the medicinal plant Coscinium fenestratum were studied to produce plant growth promotion (PGP) traits invitro. Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based on the 16S rRNA sequence, the two potent bacterial isolates, RHPR20 and MMRH22, were identified as Bacillus mojavensis and Bacillus cereus, respectively. A compatibility test between the isolates RHPR20 and MMRH22 revealed they are compatible and can be used as a consortium. Both isolates were evaluated for the plant growth promotion and the biofortification of sorghum under greenhouse conditions. Treatments included the application of MMRH22, RHPR20, their consortium (RHPR20 + MMRH22), and an uninoculated control. Inoculation with bacterial cultures resulted in a significant increase in the plant height; the number of leaves; the leaf area; the root, shoot, and leaf weight; and the yield of sorghum at 30 and 60 days after sowing (DAS). The scanning electron micrograph of the sorghum plant roots revealed extensive colonization in the plants treated with the bacterial cultures compared to the uninoculated control. The sorghum grains obtained after final harvest were analyzed for their nutrient content by ICP–OES. The biofortification in sorghum grains was varied and was found to enhance the iron content up to 97%. This study revealed that treatments with microbial consortia enhance plant growth, yield, and iron content, which could combat nutrient deficiencies in plants and humans. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Manasa, Mansani and Ravinder, Polapally and Gopalakrishnan, Subramaniam and Srinivas, Vadlamudi and Sayyed, R. Z. and El Enshasy, Hesham Ali and Yahayu, Maizatulakmal and Tan, Ali Kee Zuan and Kassem, Hazem S. and Hameeda, Bee (2021) Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum. Sustainability, 13 (21). art. no. 12091. pp. 1-15. ISSN 2071-1050 https://www.mdpi.com/2071-1050/13/21/12091 10.3390/su132112091
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Seven Bacillus spp. isolated from the marine water and the rhizosphere of the medicinal plant Coscinium fenestratum were studied to produce plant growth promotion (PGP) traits invitro. Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based on the 16S rRNA sequence, the two potent bacterial isolates, RHPR20 and MMRH22, were identified as Bacillus mojavensis and Bacillus cereus, respectively. A compatibility test between the isolates RHPR20 and MMRH22 revealed they are compatible and can be used as a consortium. Both isolates were evaluated for the plant growth promotion and the biofortification of sorghum under greenhouse conditions. Treatments included the application of MMRH22, RHPR20, their consortium (RHPR20 + MMRH22), and an uninoculated control. Inoculation with bacterial cultures resulted in a significant increase in the plant height; the number of leaves; the leaf area; the root, shoot, and leaf weight; and the yield of sorghum at 30 and 60 days after sowing (DAS). The scanning electron micrograph of the sorghum plant roots revealed extensive colonization in the plants treated with the bacterial cultures compared to the uninoculated control. The sorghum grains obtained after final harvest were analyzed for their nutrient content by ICP–OES. The biofortification in sorghum grains was varied and was found to enhance the iron content up to 97%. This study revealed that treatments with microbial consortia enhance plant growth, yield, and iron content, which could combat nutrient deficiencies in plants and humans.
format Article
author Manasa, Mansani
Ravinder, Polapally
Gopalakrishnan, Subramaniam
Srinivas, Vadlamudi
Sayyed, R. Z.
El Enshasy, Hesham Ali
Yahayu, Maizatulakmal
Tan, Ali Kee Zuan
Kassem, Hazem S.
Hameeda, Bee
spellingShingle Manasa, Mansani
Ravinder, Polapally
Gopalakrishnan, Subramaniam
Srinivas, Vadlamudi
Sayyed, R. Z.
El Enshasy, Hesham Ali
Yahayu, Maizatulakmal
Tan, Ali Kee Zuan
Kassem, Hazem S.
Hameeda, Bee
Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum
author_facet Manasa, Mansani
Ravinder, Polapally
Gopalakrishnan, Subramaniam
Srinivas, Vadlamudi
Sayyed, R. Z.
El Enshasy, Hesham Ali
Yahayu, Maizatulakmal
Tan, Ali Kee Zuan
Kassem, Hazem S.
Hameeda, Bee
author_sort Manasa, Mansani
title Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum
title_short Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum
title_full Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum
title_fullStr Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum
title_full_unstemmed Co-Inoculation of Bacillus spp. for growth promotion and iron fortification in Sorghum
title_sort co-inoculation of bacillus spp. for growth promotion and iron fortification in sorghum
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/96429/
https://www.mdpi.com/2071-1050/13/21/12091
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