Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia

The re-emergence of the Fusarium wilt caused by Fusarium odoratissimum (F. odoratissimum) causes global banana production loss. Thirty-eight isolates of Fusarium species (Fusarium spp.) were examined for morphological characteristics on different media, showing the typical Fusarium spp. The phylogen...

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Main Authors: Ujat, Anysia Hedy, Vadamalai, Ganesan, Hattori, Yukako, Nakashima, Chiharu, Wong, Clement Kiing Fook, Zulperi, Dzarifah
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96577/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/96577/
https://www.mdpi.com/2073-4395/11/10/1955
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.965772023-01-11T07:28:05Z http://psasir.upm.edu.my/id/eprint/96577/ Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia Ujat, Anysia Hedy Vadamalai, Ganesan Hattori, Yukako Nakashima, Chiharu Wong, Clement Kiing Fook Zulperi, Dzarifah The re-emergence of the Fusarium wilt caused by Fusarium odoratissimum (F. odoratissimum) causes global banana production loss. Thirty-eight isolates of Fusarium species (Fusarium spp.) were examined for morphological characteristics on different media, showing the typical Fusarium spp. The phylogenetic trees of Fusarium isolates were generated using the sequences of histone gene (H3) and translation elongation factor gene (TEF-1α). Specific primers were used to confirm the presence of F. odoratissimum. The phylogenetic trees showed the rich diversity of the genus Fusarium related to Fusarium wilt, which consists of F. odoratissimum, Fusarium grosmichelii, Fusarium sacchari, and an unknown species of the Fusarium oxysporum species complex. By using Foc-TR4 specific primers, 27 isolates were confirmed as F. odoratissimum. A pathogenicity test was conducted for 30 days on five different local cultivars including, Musa acuminata (AAA, AA) and Musa paradisiaca (AAB, ABB). Although foliar symptoms showed different severity of those disease progression, vascular symptoms of the inoculated plantlet showed that infection was uniformly severe. Therefore, it can be concluded that the Fusarium oxysporum species complex related to Fusarium wilt of banana in Malaysia is rich in diversity, and F. odoratissimum has pathogenicity to local banana cultivars in Malaysia regardless of the genotype of the banana plants. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96577/1/ABSTRACT.pdf Ujat, Anysia Hedy and Vadamalai, Ganesan and Hattori, Yukako and Nakashima, Chiharu and Wong, Clement Kiing Fook and Zulperi, Dzarifah (2021) Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia. Agronomy-Basel, 11 (10). pp. 1-16. ISSN 2073-4395 https://www.mdpi.com/2073-4395/11/10/1955 10.3390/agronomy11101955
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The re-emergence of the Fusarium wilt caused by Fusarium odoratissimum (F. odoratissimum) causes global banana production loss. Thirty-eight isolates of Fusarium species (Fusarium spp.) were examined for morphological characteristics on different media, showing the typical Fusarium spp. The phylogenetic trees of Fusarium isolates were generated using the sequences of histone gene (H3) and translation elongation factor gene (TEF-1α). Specific primers were used to confirm the presence of F. odoratissimum. The phylogenetic trees showed the rich diversity of the genus Fusarium related to Fusarium wilt, which consists of F. odoratissimum, Fusarium grosmichelii, Fusarium sacchari, and an unknown species of the Fusarium oxysporum species complex. By using Foc-TR4 specific primers, 27 isolates were confirmed as F. odoratissimum. A pathogenicity test was conducted for 30 days on five different local cultivars including, Musa acuminata (AAA, AA) and Musa paradisiaca (AAB, ABB). Although foliar symptoms showed different severity of those disease progression, vascular symptoms of the inoculated plantlet showed that infection was uniformly severe. Therefore, it can be concluded that the Fusarium oxysporum species complex related to Fusarium wilt of banana in Malaysia is rich in diversity, and F. odoratissimum has pathogenicity to local banana cultivars in Malaysia regardless of the genotype of the banana plants.
format Article
author Ujat, Anysia Hedy
Vadamalai, Ganesan
Hattori, Yukako
Nakashima, Chiharu
Wong, Clement Kiing Fook
Zulperi, Dzarifah
spellingShingle Ujat, Anysia Hedy
Vadamalai, Ganesan
Hattori, Yukako
Nakashima, Chiharu
Wong, Clement Kiing Fook
Zulperi, Dzarifah
Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia
author_facet Ujat, Anysia Hedy
Vadamalai, Ganesan
Hattori, Yukako
Nakashima, Chiharu
Wong, Clement Kiing Fook
Zulperi, Dzarifah
author_sort Ujat, Anysia Hedy
title Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia
title_short Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia
title_full Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia
title_fullStr Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia
title_full_unstemmed Current classification and diversity of Fusarium species complex, the causal pathogen of Fusarium wilt disease of banana in Malaysia
title_sort current classification and diversity of fusarium species complex, the causal pathogen of fusarium wilt disease of banana in malaysia
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/96577/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/96577/
https://www.mdpi.com/2073-4395/11/10/1955
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