Evaluation of Indonesian mangrove Xylocarpus granatum leaves ethyl acetate extract as potential anticancer drug

• Main Authors: Darmadi, Jason, Batubara, Razethy Rahayu, Himawan, Sandiego, Norma, Nur Azizah, Audah, Hilyatushalihah Kholis, Arsianti, Ade, Kurniawaty, Evi, Ismail, Intan Safinar, Batubara, Irmanida, Abdurachim Audah, Kholis
• Format: Article
• Language: English
• Published: Nature Portfolio 2021
• Online Access: http://psasir.upm.edu.my/id/eprint/97161/1/ABSTRACT.pdf; http://psasir.upm.edu.my/id/eprint/97161/; https://www.nature.com/articles/s41598-021-85383-3
• Institution: Universiti Putra Malaysia

Local Xylocarpus granatum leaves were extracted by ethyl acetate solvent and characterized by TLC fngerprinting and 2D 1 H NMR spectroscopy to contain phenolic compounds as well as several organic and amino acids as metabolic byproducts, such as succinic acid and acetic acid. Traces of favonoids and other non-categorized phenolic compounds exhibited intermediate antioxidant activity (antioxidant IC50 84.93 ppm) as well as anticancer activity against HeLa, T47D, and HT-29 cell lines; which the latter being most efective against HT-29 with Fraction 5 contained the strongest activity (anticancer IC50 23.12 ppm). Extracts also behaved as a natural growth factor and nonlethal towards brine shrimps as well as human adipose-derived stem cell hADSC due to antioxidative properties. A stability test was performed to examine how storage conditions factored in bioactivity and phytochemical structure. Extracts were compared with several studies about X. granatum leaves extracts to evaluate how ethnogeography and ecosystem factored on biologically active compounds. Further research on anticancer or antioxidant mechanism on cancer cells is needed to determine whether the extract is suitable as a candidate for an anticancer drug.