Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells

The antioxidant properties of germinated brown rice (GBR) are likely mediated by multiple bioactives. To test this hypothesis, HepG2 cells pretreated with GBR extracts, rich in acylated steryl glycoside (ASG), gamma amino butyric acid GABA), phenolics or oryzanol, were incubated with hydrogen peroxi...

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Main Authors: Nur Diyana, Md Zamri, Mustapha Umar, Imam, Maznah, Ismail, Siti Aisyah, Abd Ghafar
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2015
Subjects:
P53
Online Access:http://ddms.usim.edu.my/handle/123456789/8317
http://www.hindawi.com/journals/ecam/2014/371907/
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Institution: Universiti Sains Islam Malaysia
Language: English
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spelling my.usim-83172015-12-29T08:37:22Z Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells Nur Diyana, Md Zamri, Mustapha Umar, Imam, Maznah, Ismail, Siti Aisyah, Abd Ghafar, Survival P53 Type-2 Diabetic-Rats Induced Apoptosis The antioxidant properties of germinated brown rice (GBR) are likely mediated by multiple bioactives. To test this hypothesis, HepG2 cells pretreated with GBR extracts, rich in acylated steryl glycoside (ASG), gamma amino butyric acid GABA), phenolics or oryzanol, were incubated with hydrogen peroxide (H2O2) and their hydroxyl radical (OH center dot) scavenging capacities and thiobarbituric acid-reactive substances (TBARS) generation were evaluated. Results showed that GBR-extracts increased OH center dot scavenging activities in both cell-free medium and posttreatment culture media, suggesting that the extracts were both direct-and indirect-acting against OH center dot. The levels of TBARS in the culture medium after treatment were also reduced by all the extracts. In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Our findings show that GBR bioactive-rich extracts reduce oxidative stress through improvement in antioxidant capacity, partly mediated through transcriptional regulation of antioxidant and prosurvival genes. 2015-06-10T08:28:57Z 2015-06-10T08:28:57Z 2014-01-01 Article 1741-427X 1741-4288 http://ddms.usim.edu.my/handle/123456789/8317 http://www.hindawi.com/journals/ecam/2014/371907/ en Hindawi Publishing Corporation
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Survival
P53
Type-2 Diabetic-Rats
Induced Apoptosis
spellingShingle Survival
P53
Type-2 Diabetic-Rats
Induced Apoptosis
Nur Diyana, Md Zamri,
Mustapha Umar, Imam,
Maznah, Ismail,
Siti Aisyah, Abd Ghafar,
Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells
description The antioxidant properties of germinated brown rice (GBR) are likely mediated by multiple bioactives. To test this hypothesis, HepG2 cells pretreated with GBR extracts, rich in acylated steryl glycoside (ASG), gamma amino butyric acid GABA), phenolics or oryzanol, were incubated with hydrogen peroxide (H2O2) and their hydroxyl radical (OH center dot) scavenging capacities and thiobarbituric acid-reactive substances (TBARS) generation were evaluated. Results showed that GBR-extracts increased OH center dot scavenging activities in both cell-free medium and posttreatment culture media, suggesting that the extracts were both direct-and indirect-acting against OH center dot. The levels of TBARS in the culture medium after treatment were also reduced by all the extracts. In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Our findings show that GBR bioactive-rich extracts reduce oxidative stress through improvement in antioxidant capacity, partly mediated through transcriptional regulation of antioxidant and prosurvival genes.
format Article
author Nur Diyana, Md Zamri,
Mustapha Umar, Imam,
Maznah, Ismail,
Siti Aisyah, Abd Ghafar,
author_facet Nur Diyana, Md Zamri,
Mustapha Umar, Imam,
Maznah, Ismail,
Siti Aisyah, Abd Ghafar,
author_sort Nur Diyana, Md Zamri,
title Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells
title_short Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells
title_full Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells
title_fullStr Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells
title_full_unstemmed Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H2O2-Induced Oxidative Stress in HepG2 Cells
title_sort antioxidative effects of germinated brown rice-derived extracts on h2o2-induced oxidative stress in hepg2 cells
publisher Hindawi Publishing Corporation
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8317
http://www.hindawi.com/journals/ecam/2014/371907/
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