Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture

This investigation reports the tolerance and biodegradation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). Higher tolerance was observed with benzene (IC50 value up to 191.25 mg/L), followed b...

Full description

Saved in:
Bibliographic Details
Main Authors: E. A., Fellie, K. K., Wong, S., Salmijah, P., Sannasi, J., Kader
Format: Article
Language:en_US
Published: Research Journal Biotechnology 2015
Subjects:
TEX
Online Access:http://ddms.usim.edu.my/handle/123456789/8457
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Sains Islam Malaysia
Language: en_US
id my.usim-8457
record_format dspace
spelling my.usim-84572017-02-23T04:22:54Z Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture E. A., Fellie, K. K., Wong, S., Salmijah, P., Sannasi, J., Kader, TEX BTEX Tolerance Bacteria Bioremediation Biodegradation This investigation reports the tolerance and biodegradation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). Higher tolerance was observed with benzene (IC50 value up to 191.25 mg/L), followed by toluene (IC50 = 139.67 mg/L), xylene (IC50 = 97.04 mg/L) and ethylbenzene (IC50 = 96.99 mg/L). Significant decrease (p < 0.05) in the specific growth rate (mu), however was observed as the concentrations of each individual BTEX were increased from 10 mg/L to 500 mg/L. Growth of CC was completely inhibited at 250 mg/L ethylbenzene and 500 mg/L xylene. Toxicity followed the trend: B<T<X<E. Biodegradation of individual BTEX compound was monitored by gas chromatography. The GC-FID chromatographic profiles showed the capability of CC to significantly biodegrade (p < 0.05) benzene (61.66 %), toluene (55.91 %), ethylbenzene (37.15 %), p-xylene (43.66 %), m-xylene (47.86 %) and o-xylene (41.03 %) at an initial concentration of 50 mg/L after 48 hours. These findings confirm the ability of CC to withstand biodegrade and utilize BTEX as the sole source of carbon and energy in the following order: B>T>X>E. 2015-06-19T06:40:44Z 2015-06-19T06:40:44Z 2012 Article 0973-6263 http://ddms.usim.edu.my/handle/123456789/8457 en_US Research Journal Biotechnology
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
topic TEX
BTEX Tolerance
Bacteria
Bioremediation
Biodegradation
spellingShingle TEX
BTEX Tolerance
Bacteria
Bioremediation
Biodegradation
E. A., Fellie,
K. K., Wong,
S., Salmijah,
P., Sannasi,
J., Kader,
Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture
description This investigation reports the tolerance and biodegradation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). Higher tolerance was observed with benzene (IC50 value up to 191.25 mg/L), followed by toluene (IC50 = 139.67 mg/L), xylene (IC50 = 97.04 mg/L) and ethylbenzene (IC50 = 96.99 mg/L). Significant decrease (p < 0.05) in the specific growth rate (mu), however was observed as the concentrations of each individual BTEX were increased from 10 mg/L to 500 mg/L. Growth of CC was completely inhibited at 250 mg/L ethylbenzene and 500 mg/L xylene. Toxicity followed the trend: B<T<X<E. Biodegradation of individual BTEX compound was monitored by gas chromatography. The GC-FID chromatographic profiles showed the capability of CC to significantly biodegrade (p < 0.05) benzene (61.66 %), toluene (55.91 %), ethylbenzene (37.15 %), p-xylene (43.66 %), m-xylene (47.86 %) and o-xylene (41.03 %) at an initial concentration of 50 mg/L after 48 hours. These findings confirm the ability of CC to withstand biodegrade and utilize BTEX as the sole source of carbon and energy in the following order: B>T>X>E.
format Article
author E. A., Fellie,
K. K., Wong,
S., Salmijah,
P., Sannasi,
J., Kader,
author_facet E. A., Fellie,
K. K., Wong,
S., Salmijah,
P., Sannasi,
J., Kader,
author_sort E. A., Fellie,
title Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture
title_short Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture
title_full Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture
title_fullStr Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture
title_full_unstemmed Tolerance and Biodegradation of Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) by a Metal Acclimatized Bacterial Consortium Culture
title_sort tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (btex) by a metal acclimatized bacterial consortium culture
publisher Research Journal Biotechnology
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8457
_version_ 1645152420813078528