Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes

• Main Author: Foong , Choon Pin
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: QH1 Natural history (General - Including nature conservation, geographical distribution)
• Online Access: http://eprints.usm.my/31368/1/FOONG_CHOON_PIN_24.pdf; http://eprints.usm.my/31368/
• Institution: Universiti Sains Malaysia

Komuniti mikrob bagi dua tanah paya bakau Pulau Pinang (Batu Maung dan Balik Pulau) yang dipengaruhi oleh aktiviti antropogenik telah dikaji dengan menggunakan pendekatan penjujukan metagenomik “shotgun” tanpa-kultur. Dua set data metagenomik (~250 GB) dihasilkan melalui platfom “Next-generation Sequencing (NGS)” Illumina HiSeq dan disimpan dalam pelayan awam “Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST)”. Analisis taksonomi mikrob menunjukkan bahawa kedua-dua tanah paya bakau Pulau Pinang didominasi oleh Bakteria (97 %), Proteobakteria (43 %) dan Deltaproteobakteria (15 %) pada peringkat domain,. filum dan kelas masing-masing. Pada peringkat genus, kebanyakan bakteria anaerobik diperhatikan terdiri daripada Deltaproteobakteria. Sebahagian besar daripada jujukan adalah milik spesis mikrob (70 %) dan filum (32 %) yang belum dikenalpasti atau belum dikultur. The microbial communities of two local Penang mangrove soils (Batu Maung and Balik Pulau) which are under anthropogenic influences were investigated using culture-independent shotgun metagenome sequencing approach. Two metagenome data sets (~250 GB) were generated from the Illumina HiSeq next-generation sequencing (NGS) platform and then deposited in Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST) public server. Microbial taxonomic analysis showed that both Penang mangrove soils were dominated by Bacteria (97 %), Proteobacteria (43 %) and Deltaproteobacteria (15 %) at the domain, phylum and class levels, respectively. At the genus level, predominance of anaerobic bacteria was observed and mostly belonged to Deltaproteobacteria. A large portion of the reads belonged to unknown or yet uncultured microbial species (70 %) and microbial phyla (32 %).