Physicochemical Properties And Prebiotic Potential Of Native, Resistant And Hcl-Resistant Starches From Sago (Metroxylon Sagu)

• Main Author: Tan, Zi Ni
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: T1-995 Technology(General)
• Online Access: http://eprints.usm.my/31864/1/TAN_ZI_NI_24%28NN%29.pdf; http://eprints.usm.my/31864/
• Institution: Universiti Sains Malaysia

Kanji rintang jenis III (RS3) telah dihasilkan daripada sagu (Metroxylon sagu) dan dinilai sifat fizikokimia and potensinya sebagai prebiotik. Sampel mengandungi 35.7% kanji rintang (dikenal sebagai sagu RS) telah dihasilkan apabila kanji sagu asli diautoklaf dalam air suling pada suhu 121 °C selama 1 jam, dinyahcabang dengan 20 U pullulanase per g kanji pada 60 °C selama 24 jam dan seterusnya diautoklaf sekali lagi pada 121 °C selama 1 jam sebelum disimpan pada 4 °C selama 24 jam. Seterusnya, kandungan kanji rintang meningkat sehingga 63.8% (sampel dikenal sebagai HCl-sagu RS) selepas sagu RS dihidrolisiskan dengan 0.5 M HCl pada suhu 60 °C. Granul sagu RS dan HCl-sagu RS menunjukkan corak pembelauan sinar X jenis B, suhu puncak yang tinggi (143.7 °C and 146.5 ºC, masing-masing) dan struktur permukaan yang tidak sekata dan kasar. Granul kanji sagu asli menunjukkan corak pembelauan sina X jenis C, suhu puncak 74.6 °C dan permukaan yang sekata. Keterlarutan dan kuasa pembengkakan sampel HCl-sagu RS ialah 14.9% dan 1.94 g/g, masing-masing, iaitu lebih rendah berbanding sagu RS (27.4% and 2.82 g/g, masing-masing). Resistant starch type III (RS3) was produced from sago (Metroxylon sagu) and evaluated for its physicochemical properties and potential as a prebiotic. A sample with 35.7% RS3 content (designated as sago RS) was produced when the native sago starch was suspended in distilled water, gelatinized by autoclaving at 121 °C for 1 h, followed by debranching with 20 U pullulanase per g starch at 60 °C for 24 h, autoclaved again at 121 °C for 1 h before storage at 4 °C for 24 h. RS3 content was further increased with the treatment of sago RS with 0.5 M HCl at 60 °C (sample designated HCl-sago RS) to 63.8%. Granules of sago RS and HCl-sago RS had B-type X-ray diffraction pattern, high peak temperatures (143.7 °C and 146.5 ºC, respectively) and showed irregular and rough surface structure. While granules of native sago starch had C-type diffraction pattern, peak temperature of 74.6 °C and smooth granular surface. The solubility and the swelling power of HCl-sago RS samples were 14.9% and 1.94 g/g, respectively, which were lower than that of sago RS (27.4% and 2.82 g/g, respectively).