Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line

Green synthesis is preferable for the synthesis of silver nanoparticles (AgNPs) due to its rapid and reliable process. Previous studies have demonstrated that active compounds present in Vernonia cinerea (V. cinerea) are responsible for bioreduction during synthesis of AgNPs. Hence, in this present...

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Main Authors: Mohamad Zani, Radhiyatul Akma, Ahmad, Nor Hazwani, Abdul Razak, Siti Razila
Format: Article
Language:English
Published: Universiti Teknologi Malaysia 2016
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Online Access:http://eprints.usm.my/37711/1/9873-27670-2-PB.pdf
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spelling my.usm.eprints.37711 http://eprints.usm.my/37711/ Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line Mohamad Zani, Radhiyatul Akma Ahmad, Nor Hazwani Abdul Razak, Siti Razila RV Botanic, Thomsonian, and eclectic medicine Green synthesis is preferable for the synthesis of silver nanoparticles (AgNPs) due to its rapid and reliable process. Previous studies have demonstrated that active compounds present in Vernonia cinerea (V. cinerea) are responsible for bioreduction during synthesis of AgNPs. Hence, in this present study, aqueous extract of V. cinerea was used to synthesise AgNPs. The V. cinerea aqueous extract was added to AgNO3 solution (25 mL, 50 mL and 100 mL) to produce mixture with ratio of 1:5, 1:10 and 1:20. The formation of V. cinerea-AgNPs was monitored for colour changes, and characterised by UV-Vis spectroscopy, transmission electron microscopy (TEM) and zeta potential analyses. The UV-Vis spectrum of V. cinerea-AgNPs showed absorption maxima at 450 nm while TEM images showed that the V. cinerea-AgNPs were spherical in shape with an average size of 15.29 nm. The zeta value of synthesised V. cinerea-AgNPs was – 29.50 mV with a peak area of 100 % intensity. The cytotoxicity of V. cinerea-AgNPs were tested in vitro on Kasumi-1 cells using MTS assay. The MTS assay revealed that the V. cinerea-AgNPs with the concentration of 31.25 g/mL at 72 hr produced highest cytotoxicity effects on Kasumi-1 cells. These results indicate that AgNPs can be biologically synthesised from V. cinerea and has potential to be used as an anticancer agents on acute myeloid leukaemia cell line Universiti Teknologi Malaysia 2016 Article PeerReviewed application/pdf en cc_by http://eprints.usm.my/37711/1/9873-27670-2-PB.pdf Mohamad Zani, Radhiyatul Akma and Ahmad, Nor Hazwani and Abdul Razak, Siti Razila (2016) Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line. Jurnal Teknologi, 78 (11-3). pp. 57-65. ISSN 0127-9696 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/9873
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RV Botanic, Thomsonian, and eclectic medicine
spellingShingle RV Botanic, Thomsonian, and eclectic medicine
Mohamad Zani, Radhiyatul Akma
Ahmad, Nor Hazwani
Abdul Razak, Siti Razila
Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line
description Green synthesis is preferable for the synthesis of silver nanoparticles (AgNPs) due to its rapid and reliable process. Previous studies have demonstrated that active compounds present in Vernonia cinerea (V. cinerea) are responsible for bioreduction during synthesis of AgNPs. Hence, in this present study, aqueous extract of V. cinerea was used to synthesise AgNPs. The V. cinerea aqueous extract was added to AgNO3 solution (25 mL, 50 mL and 100 mL) to produce mixture with ratio of 1:5, 1:10 and 1:20. The formation of V. cinerea-AgNPs was monitored for colour changes, and characterised by UV-Vis spectroscopy, transmission electron microscopy (TEM) and zeta potential analyses. The UV-Vis spectrum of V. cinerea-AgNPs showed absorption maxima at 450 nm while TEM images showed that the V. cinerea-AgNPs were spherical in shape with an average size of 15.29 nm. The zeta value of synthesised V. cinerea-AgNPs was – 29.50 mV with a peak area of 100 % intensity. The cytotoxicity of V. cinerea-AgNPs were tested in vitro on Kasumi-1 cells using MTS assay. The MTS assay revealed that the V. cinerea-AgNPs with the concentration of 31.25 g/mL at 72 hr produced highest cytotoxicity effects on Kasumi-1 cells. These results indicate that AgNPs can be biologically synthesised from V. cinerea and has potential to be used as an anticancer agents on acute myeloid leukaemia cell line
format Article
author Mohamad Zani, Radhiyatul Akma
Ahmad, Nor Hazwani
Abdul Razak, Siti Razila
author_facet Mohamad Zani, Radhiyatul Akma
Ahmad, Nor Hazwani
Abdul Razak, Siti Razila
author_sort Mohamad Zani, Radhiyatul Akma
title Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line
title_short Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line
title_full Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line
title_fullStr Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line
title_full_unstemmed Synthesis and Characterisation of Silver Nanoparticles Using Vernonia Cinerea Aqueous Extract and Their Cytotoxicity Activity against Kasumi-1 Cell Line
title_sort synthesis and characterisation of silver nanoparticles using vernonia cinerea aqueous extract and their cytotoxicity activity against kasumi-1 cell line
publisher Universiti Teknologi Malaysia
publishDate 2016
url http://eprints.usm.my/37711/1/9873-27670-2-PB.pdf
http://eprints.usm.my/37711/
http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/9873
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