Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency

• Main Authors: Gnasekaran, Pavallekoodi, Antony, Jessica Jeyanthi James, Mohammad, Jasim Uddain, Subramaniam, Sreeramanan
• Format: Article
• Language: English
• Published: Hindawi Publishing Corporation 2014
• Subjects: QH1 Natural history (General - Including nature conservation, geographical distribution)
• Online Access: http://eprints.usm.my/38273/1/Agrobacterium-Mediated_Transformation_of_the_Recalcitrant_Vanda_Kasem%27s_Delight_Orchid_with.pdf; http://eprints.usm.my/38273/; http://dx.doi.org/10.1155/2014/583934
• Institution: Universiti Sains Malaysia

The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem’s Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultiv...