Cryopreservation of PLBs of Brassidium Fly Away Using Encapsulation-Dehydration Techniqu

• Main Authors: Rajasegar, Arulvilee, Poobathy, Ranjetta, Rathinam, Xavier, Oyunbileg, Yungeree, Subramaniam, Sreeramanan
• Format: Article
• Language: English
• Published: National University of Mongolia 2015
• Subjects: QH1 Natural history (General - Including nature conservation, geographical distribution)
• Online Access: http://eprints.usm.my/38552/1/Cryopreservation_of_PLBs_of_Brassidium_Fly_Away_Using.pdf; http://eprints.usm.my/38552/; http://dx.doi.org/10.22353/mjbs.2015.13.03
• Institution: Universiti Sains Malaysia

In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid were cryopreserved using encapsulation- dehydration technique. The viability of the cryopreserved cells was determined by 2,3,5-triphenyltetrazolium chloride (TTC) assay. For the preculture treatment, the PLBs were e...