Identification of a potential marker for Brugia malayi infection by Western Blot analysis

Brugia malayi adult antigen was electrophoresed on SDS-PAGE gels and electrophoretically transferred onto PVDF membranes. The membrane strips were incubated with different categories of human sera, followed by successive incubations with blocking solution, monoclonal anti-human IgG4 antibody and per...

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Main Authors:	N., Rahmah, A., Noor A'shikin, R., Mehdi, B., Abdullah, A., Khairul Anuar
Format:	Conference or Workshop Item
Language:	English
Subjects:	RA643-645 Disease (Communicable and noninfectious) and public health
Online Access:	http://eprints.usm.my/42634/1/GP...Identification_Of_A_Potential_Marker_For_Brugia_Malayi_Infection_By_Western_Blot_Analysis..OCR...pdf http://eprints.usm.my/42634/
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Institution:	Universiti Sains Malaysia
Language:	English

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spelling	my.usm.eprints.42634 http://eprints.usm.my/42634/ Identification of a potential marker for Brugia malayi infection by Western Blot analysis N., Rahmah A., Noor A'shikin R., Mehdi B., Abdullah A., Khairul Anuar RA643-645 Disease (Communicable and noninfectious) and public health Brugia malayi adult antigen was electrophoresed on SDS-PAGE gels and electrophoretically transferred onto PVDF membranes. The membrane strips were incubated with different categories of human sera, followed by successive incubations with blocking solution, monoclonal anti-human IgG4 antibody and peroxidase rabbit anti-mouse IgG antibody; with adequate washings done in between each incubation steps. Chemiluminescence detection was used to develop the blots. Two antigenic epitopes (molecular weights of -67-68 kDa and -54-55 kDA) were found to be present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with positive anti-filarial IgG4 antibodies, some treated patients and some elephantiasis patients. We hypothesized that last two groups are still harbouring live adult worms despite being treated or in the chronic stage respectively. The two epitopes did not simultaneously react with soil-transmitted helminth sera, normal endemic sera and sera of city dwellers.Therefore the simultaneous presence of both epitopes may potentially serve as a marker for Brugia malayi infection. Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.usm.my/42634/1/GP...Identification_Of_A_Potential_Marker_For_Brugia_Malayi_Infection_By_Western_Blot_Analysis..OCR...pdf N., Rahmah and A., Noor A'shikin and R., Mehdi and B., Abdullah and A., Khairul Anuar Identification of a potential marker for Brugia malayi infection by Western Blot analysis. In: Identification of a potential marker for Brugia malayi infection by Western Blot analysis. (Submitted)
institution	Universiti Sains Malaysia
building	Hamzah Sendut Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sains Malaysia
content_source	USM Institutional Repository
url_provider	http://eprints.usm.my/
language	English
topic	RA643-645 Disease (Communicable and noninfectious) and public health
spellingShingle	RA643-645 Disease (Communicable and noninfectious) and public health N., Rahmah A., Noor A'shikin R., Mehdi B., Abdullah A., Khairul Anuar Identification of a potential marker for Brugia malayi infection by Western Blot analysis
description	Brugia malayi adult antigen was electrophoresed on SDS-PAGE gels and electrophoretically transferred onto PVDF membranes. The membrane strips were incubated with different categories of human sera, followed by successive incubations with blocking solution, monoclonal anti-human IgG4 antibody and peroxidase rabbit anti-mouse IgG antibody; with adequate washings done in between each incubation steps. Chemiluminescence detection was used to develop the blots. Two antigenic epitopes (molecular weights of -67-68 kDa and -54-55 kDA) were found to be present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with positive anti-filarial IgG4 antibodies, some treated patients and some elephantiasis patients. We hypothesized that last two groups are still harbouring live adult worms despite being treated or in the chronic stage respectively. The two epitopes did not simultaneously react with soil-transmitted helminth sera, normal endemic sera and sera of city dwellers.Therefore the simultaneous presence of both epitopes may potentially serve as a marker for Brugia malayi infection.
format	Conference or Workshop Item
author	N., Rahmah A., Noor A'shikin R., Mehdi B., Abdullah A., Khairul Anuar
author_facet	N., Rahmah A., Noor A'shikin R., Mehdi B., Abdullah A., Khairul Anuar
author_sort	N., Rahmah
title	Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_short	Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_full	Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_fullStr	Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_full_unstemmed	Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_sort	identification of a potential marker for brugia malayi infection by western blot analysis
url	http://eprints.usm.my/42634/1/GP...Identification_Of_A_Potential_Marker_For_Brugia_Malayi_Infection_By_Western_Blot_Analysis..OCR...pdf http://eprints.usm.my/42634/
_version_	1662755731517997056

Identification of a potential marker for Brugia malayi infection by Western Blot analysis

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