The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells

The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells

D6 and DARC had been reported as a decoy chemokine receptor in cancer study. The involvement of D6 and DARC in breast cancer had been investigated and it was reported to negatively correlate with the progression and metastasis of breast cancer cells. This study aimed to construct recombinant clones...

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Main Author: Tan, Wee Yee
Format: Thesis
Language:English
Published: 2018
Subjects:
R735-854 Medical education. Medical schools. Research
Online Access:http://eprints.usm.my/44025/1/TAN%20WEE%20YEE.pdf
http://eprints.usm.my/44025/
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Institution: Universiti Sains Malaysia
Language: English
id my.usm.eprints.44025
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spelling my.usm.eprints.44025 http://eprints.usm.my/44025/ The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells Tan, Wee Yee R735-854 Medical education. Medical schools. Research D6 and DARC had been reported as a decoy chemokine receptor in cancer study. The involvement of D6 and DARC in breast cancer had been investigated and it was reported to negatively correlate with the progression and metastasis of breast cancer cells. This study aimed to construct recombinant clones of D6 and DARC, express, analyze and purify the proteins and then determine the effects of the recombinant proteins on breast cancer cell migration and invasion. D6 and DARC genes in MDA-MB-231 cell line were first amplified by one-step RT-PCR with gene specific primers and Pfu DNA polymerase. Besides, DARC was also amplified by PCR using Phusion DNA polymerase. For each of the clones, two pairs of specific primers were used to generate full length nucleotide sequences which were cloned into pPICZ and pPICZα expression vectors. 2018-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/44025/1/TAN%20WEE%20YEE.pdf Tan, Wee Yee (2018) The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells. PhD thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R735-854 Medical education. Medical schools. Research
spellingShingle R735-854 Medical education. Medical schools. Research
Tan, Wee Yee
The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
description D6 and DARC had been reported as a decoy chemokine receptor in cancer study. The involvement of D6 and DARC in breast cancer had been investigated and it was reported to negatively correlate with the progression and metastasis of breast cancer cells. This study aimed to construct recombinant clones of D6 and DARC, express, analyze and purify the proteins and then determine the effects of the recombinant proteins on breast cancer cell migration and invasion. D6 and DARC genes in MDA-MB-231 cell line were first amplified by one-step RT-PCR with gene specific primers and Pfu DNA polymerase. Besides, DARC was also amplified by PCR using Phusion DNA polymerase. For each of the clones, two pairs of specific primers were used to generate full length nucleotide sequences which were cloned into pPICZ and pPICZα expression vectors.
format Thesis
author Tan, Wee Yee
author_facet Tan, Wee Yee
author_sort Tan, Wee Yee
title The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_short The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_full The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_fullStr The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_full_unstemmed The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_sort production and purification of d6 and darc chemokine decoy receptor recombinant proteins and their effects on migration and invasion in mda-mb-231 and mcf-7 cells
publishDate 2018
url http://eprints.usm.my/44025/1/TAN%20WEE%20YEE.pdf
http://eprints.usm.my/44025/
_version_ 1643710897095442432

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