Association Analysis Of Single Nucleotide Polymorphism Of Opioid Dependence Genes Among Malay Males In Malaysia

• Main Author: Nagaya, Devaki
• Format: Thesis
• Language: English
• Published: 2018
• Subjects: R Medicine; RM300-666 Drugs and their actions
• Online Access: http://eprints.usm.my/48494/1/DEVAKI%20NAGAYA_hj.pdf; http://eprints.usm.my/48494/
• Institution: Universiti Sains Malaysia

Drug addiction is a chronic and relapsing disorder is associated with genetics. There are many studies have been reported the roles of single nucleotide polymorphisms (SNPs) with drug dependence and among those, twelve (12) candidate genes that were associated with drug dependence will be investigated in this study. The candidate genes are OPRD1 (Delta Opioid Receptor), OPRK1 (Kappa Opioid Receptor), COMT (Catechol – O-Methyltransferase gene), PDYN (Prodynorphin), DRD4 (Dopamine receptor D4), ABCB1(P glycoprotein), DUSP (Dual Specificity Phosphatase 27) and rs10494334. The goal of this study was to determine the frequencies of these SNPs rs1042114, rs702764, rs199774, rs1022563, rs910080, rs737866, rs10494334, rs1800955, rs1128503, rs1045642, and rs2032582 and to study their association with opioid dependence in Malay males in Malaysian population. This genetic database will be established for pharmacogenetics-based treatment. Research participants were 459 Malay males with opioid dependence and 543 healthy Malay males as controls. SNPs were genotyped using TaqMan SNP genotyping assay. Statistical analysis was performed using Golden Helix SVS software suite to identify the distribution of allele and genotype frequencies and SNP–SNP interactions.