Quaternary Aqueous Biphasis Mixture For Enzyme Extraction From Banana
Enzyme extraction by using various solvent such as ionic liquids was used in the past. Recently, a green solvent which is deep eutectic solvents (DESs) have gained attention from the community because of its simplicity and the affordable cost in extracting the protein with aqueous two-phase syste...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2022
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| Subjects: | |
| Online Access: | http://eprints.usm.my/55124/1/Quaternary%20Aqueous%20Biphasis%20Mixture%20For%20Enzyme%20Extraction%20From%20Banana.pdf http://eprints.usm.my/55124/ |
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| Institution: | Universiti Sains Malaysia |
| Language: | English |
| Summary: | Enzyme extraction by using various solvent such as ionic liquids was used in
the past. Recently, a green solvent which is deep eutectic solvents (DESs) have gained
attention from the community because of its simplicity and the affordable cost in
extracting the protein with aqueous two-phase system (ATPS). Through this study,
four choline chloride-(HBDs) DES with different molar ratio was studied to extract
bovine serum albumin (BSA) and protein in banana. Molar ratio for choline chloride:
glycerol (ChCl: Gly) at 1:2, choline chloride: lactic acid (ChCl: Lac) at 1:2, choline
chloride: urea (ChCl: Urea) at 1:2 and choline chloride: glucose (ChCl: Glu) at 2:1
will form a clear and transparent homogenous mixture at room temperature. With the
formation of DES, the effect of the concentration and volume for polyethylene glycol
(PEG), sodium sulphate (Na2SO4) and DES were studied. 1000 μL of 1.0 M Na2SO4,
1000 μL of PEG and 100 μL of DES was chosen because it took the shortest time for
the formation of two-phase which is 97.33 mins. A calibration curve of different DES
with BSA reagent which the corrected absorbance against protein concentration is then
plotted. The protein concentration of banana was compared with the calibration curve
with different DES. The results showed ATPS with ChCl: Lac (top phase) extracted
the most protein which is 3678.33 μg/ml with the highest extraction efficiency of
89.60 %. This might be affected by the functional group of the HBDs, and low
viscosity of banana crude extract interacts with the DES where the hydrogen bonds
affect the mobility of free molecules within the system such as the movement of
protein within the solvent leads to fast mass transfer of protein. |
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