Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela
Cervical cancer is one of the leading causes of death among women worldwide. Unfortunately, most of the available cancer treatments reported to cause side effects. Therefore, patients turn to alternative treatments by utilizing natural products. Gallic acid (3,4,5‑trihydroxy benzoic acid; GA), a...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | http://eprints.usm.my/60246/1/Batrisyia-E.pdf http://eprints.usm.my/60246/ |
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| Institution: | Universiti Sains Malaysia |
| Language: | English |
| Summary: | Cervical cancer is one of the leading causes of death among women worldwide. Unfortunately,
most of the available cancer treatments reported to cause side effects. Therefore, patients turn
to alternative treatments by utilizing natural products. Gallic acid (3,4,5‑trihydroxy benzoic
acid; GA), a plant‑derived natural phenolic compound, has been reported to prevent the
development and progression of various types of cancers. Anticancer activities of GA were
reported due to regulation of reactive oxygen species (ROS) and glutathione (GSH) at the
cellular level. However, its mechanism of action is still unclear. Thus, this study aimed to
evaluate the regulations of ROS and GSH on the combination of cisplatin and GA in HeLa
cells at 24 and 48 hours treatment period at IC50 concentration. ROS levels, including H2O2
concentrations were measured in HeLa cells as these variations appear to influence cervical
cancer's vulnerability to cisplatin treatment over both the 24-hour and 48-hour intervals.
Moreover, GSH levels were measured in treated HeLa cells as it helps to provide information
about the intricate balance between the body's attempts to counter oxidative stress and the
response of cervical cancer cells to the damage caused by the treatment. The results revealed
that the combination of cisplatin and GA led to distinct patterns of ROS production for both
the 24-hour and 48-hour intervals. This highlights the substantial influence of ROS levels on
the treatment efficacy. Additionally, H2O2 levels was elevated during the 48-hour period when
cisplatin and GA were combined. This increase aligned with the initiation of apoptosis,
highlighting the time-dependent nature of H2O2 levels production, as the combination treatment
demonstrated increased concentration after 48 hours. Moreover, during the 48-hour treatment |
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