Role of horseradish peroxidase in polypyrrole conductivity

Immobilise enzymes can offer many advantages over their soluble forms making this is a topic of active research in enzyme technology for industrial applications. There are various methods for enzyme immobilization as well as support materials. The methods and supports used for immobilisation of cert...

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Main Authors: Othman, Siti Amira, Radiman, Shahidan
Format: Article
Language:English
Published: Trans Tech Publications 2017
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Online Access:http://eprints.uthm.edu.my/3580/1/AJ%202017%20%28493%29.pdf
http://eprints.uthm.edu.my/3580/
http://dx.doi.org/10.4028/www.scientific.net/SSP.268.370
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Institution: Universiti Tun Hussein Onn Malaysia
Language: English
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spelling my.uthm.eprints.35802021-11-21T02:47:24Z http://eprints.uthm.edu.my/3580/ Role of horseradish peroxidase in polypyrrole conductivity Othman, Siti Amira Radiman, Shahidan TP200-248 Chemicals: Manufacture, use, etc. Immobilise enzymes can offer many advantages over their soluble forms making this is a topic of active research in enzyme technology for industrial applications. There are various methods for enzyme immobilization as well as support materials. The methods and supports used for immobilisation of certain enzyme are chosen to ensure the highest retention of activity and its stability. Enzyme Horseradish peroxidise (HRP) was used extensively in molecular biology applications primarily for its ability to amplify a weak signal and increase the detectability of a target molecules. Recently class of conducting polymers are used as a polymeric support. Among the family a conducting polymer, polypyrrole (PPY) has intensively been used for various reason because of its unique properties such as ease of synthesis by chemical or electrochemical oxidative polymerization of the monomers, role as a polymeric support and also have potential in various field . It has alternating single and double carbon-carbon bonds along the polymeric chains. The highly conjugated polymer chain can be assigned reversible chemical, electrochemical and physical properties controlled by a doping/ de-doping process, which makes this polymers very attractive as transducer materials in various sensing devices. This paper reports the process of immobilised enzymes on polymeric substrate and the role of enzymes in PPY conductivity. The characterization was done using UV-Visible, FTIR and four point probe Trans Tech Publications 2017-10 Article PeerReviewed text en http://eprints.uthm.edu.my/3580/1/AJ%202017%20%28493%29.pdf Othman, Siti Amira and Radiman, Shahidan (2017) Role of horseradish peroxidase in polypyrrole conductivity. Solid State Phenomena, 268 (NIL). pp. 370-373. ISSN 1662-9779 http://dx.doi.org/10.4028/www.scientific.net/SSP.268.370
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
topic TP200-248 Chemicals: Manufacture, use, etc.
spellingShingle TP200-248 Chemicals: Manufacture, use, etc.
Othman, Siti Amira
Radiman, Shahidan
Role of horseradish peroxidase in polypyrrole conductivity
description Immobilise enzymes can offer many advantages over their soluble forms making this is a topic of active research in enzyme technology for industrial applications. There are various methods for enzyme immobilization as well as support materials. The methods and supports used for immobilisation of certain enzyme are chosen to ensure the highest retention of activity and its stability. Enzyme Horseradish peroxidise (HRP) was used extensively in molecular biology applications primarily for its ability to amplify a weak signal and increase the detectability of a target molecules. Recently class of conducting polymers are used as a polymeric support. Among the family a conducting polymer, polypyrrole (PPY) has intensively been used for various reason because of its unique properties such as ease of synthesis by chemical or electrochemical oxidative polymerization of the monomers, role as a polymeric support and also have potential in various field . It has alternating single and double carbon-carbon bonds along the polymeric chains. The highly conjugated polymer chain can be assigned reversible chemical, electrochemical and physical properties controlled by a doping/ de-doping process, which makes this polymers very attractive as transducer materials in various sensing devices. This paper reports the process of immobilised enzymes on polymeric substrate and the role of enzymes in PPY conductivity. The characterization was done using UV-Visible, FTIR and four point probe
format Article
author Othman, Siti Amira
Radiman, Shahidan
author_facet Othman, Siti Amira
Radiman, Shahidan
author_sort Othman, Siti Amira
title Role of horseradish peroxidase in polypyrrole conductivity
title_short Role of horseradish peroxidase in polypyrrole conductivity
title_full Role of horseradish peroxidase in polypyrrole conductivity
title_fullStr Role of horseradish peroxidase in polypyrrole conductivity
title_full_unstemmed Role of horseradish peroxidase in polypyrrole conductivity
title_sort role of horseradish peroxidase in polypyrrole conductivity
publisher Trans Tech Publications
publishDate 2017
url http://eprints.uthm.edu.my/3580/1/AJ%202017%20%28493%29.pdf
http://eprints.uthm.edu.my/3580/
http://dx.doi.org/10.4028/www.scientific.net/SSP.268.370
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