Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support
Enzyme immobilization on inorganic materials is gaining more attention with the potential characteristics of high-surface-area-to-volume ratios, increasing the efficiency of enzyme loading on the support. Metal oxide hybrid support was prepared by a wetness impregnation of five metal precursors, inc...
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my.utm.1013442023-06-08T09:34:47Z http://eprints.utm.my/id/eprint/101344/ Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support Ramlee, Nurfadhila Nasya Md. Illias, Rosli A. Rahman, Roshanida Toemen, Susilawati Selvasembian, Rangabhashiyam Ahmad, Rabi’atul Adawiyah Abdul Manas, Nor Hasmaliana Wan Azelee, Nur Izyan TP Chemical technology Enzyme immobilization on inorganic materials is gaining more attention with the potential characteristics of high-surface-area-to-volume ratios, increasing the efficiency of enzyme loading on the support. Metal oxide hybrid support was prepared by a wetness impregnation of five metal precursors, including CaO, CuO, MgO, NiO, and ZnO, on Al2O3 and used as a support for the immobilization of Candida rugosa lipase (CRL) by adsorption. Maximum activity recovery (70.6%) and immobilization efficiency (63.2%) were obtained after optimization of five parameters using response surface methodology (RSM) by Box–Behnken design (BBD). The biochemical properties of immobilized CRL showed high thermostability up to 70 °C and a wide range in pH stability (pH 4–10). TGA-DTA and FTIR analysis were conducted, verifying thermo-decomposition of lipase and the presence of an amide bond. FESEM-EDX showed the homogeneous distribution and high dispersion of magnesium and CRL on MgO-Al2O3, while a nitrogen adsorption–desorption study confirmed MgO-Al2O3 as a mesoporous material. CRL/MgO-Al2O3 can be reused for up to 12 cycles and it demonstrated high tolerance in solvents (ethanol, isopropanol, methanol, and tert-butanol) compared to free CRL. MDPI 2022 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/101344/1/NurIzyanWanAzelee2022_BiochemicalandPhysicalCharacterizationofImmobilizedCandidaRugosa.pdf Ramlee, Nurfadhila Nasya and Md. Illias, Rosli and A. Rahman, Roshanida and Toemen, Susilawati and Selvasembian, Rangabhashiyam and Ahmad, Rabi’atul Adawiyah and Abdul Manas, Nor Hasmaliana and Wan Azelee, Nur Izyan (2022) Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support. Catalysts, 12 (8). pp. 1-32. ISSN 2073-4344 http://dx.doi.org/10.3390/catal12080854 DOI : 10.3390/catal12080854 |
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TP Chemical technology Ramlee, Nurfadhila Nasya Md. Illias, Rosli A. Rahman, Roshanida Toemen, Susilawati Selvasembian, Rangabhashiyam Ahmad, Rabi’atul Adawiyah Abdul Manas, Nor Hasmaliana Wan Azelee, Nur Izyan Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support |
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Enzyme immobilization on inorganic materials is gaining more attention with the potential characteristics of high-surface-area-to-volume ratios, increasing the efficiency of enzyme loading on the support. Metal oxide hybrid support was prepared by a wetness impregnation of five metal precursors, including CaO, CuO, MgO, NiO, and ZnO, on Al2O3 and used as a support for the immobilization of Candida rugosa lipase (CRL) by adsorption. Maximum activity recovery (70.6%) and immobilization efficiency (63.2%) were obtained after optimization of five parameters using response surface methodology (RSM) by Box–Behnken design (BBD). The biochemical properties of immobilized CRL showed high thermostability up to 70 °C and a wide range in pH stability (pH 4–10). TGA-DTA and FTIR analysis were conducted, verifying thermo-decomposition of lipase and the presence of an amide bond. FESEM-EDX showed the homogeneous distribution and high dispersion of magnesium and CRL on MgO-Al2O3, while a nitrogen adsorption–desorption study confirmed MgO-Al2O3 as a mesoporous material. CRL/MgO-Al2O3 can be reused for up to 12 cycles and it demonstrated high tolerance in solvents (ethanol, isopropanol, methanol, and tert-butanol) compared to free CRL. |
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Article |
author |
Ramlee, Nurfadhila Nasya Md. Illias, Rosli A. Rahman, Roshanida Toemen, Susilawati Selvasembian, Rangabhashiyam Ahmad, Rabi’atul Adawiyah Abdul Manas, Nor Hasmaliana Wan Azelee, Nur Izyan |
author_facet |
Ramlee, Nurfadhila Nasya Md. Illias, Rosli A. Rahman, Roshanida Toemen, Susilawati Selvasembian, Rangabhashiyam Ahmad, Rabi’atul Adawiyah Abdul Manas, Nor Hasmaliana Wan Azelee, Nur Izyan |
author_sort |
Ramlee, Nurfadhila Nasya |
title |
Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support |
title_short |
Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support |
title_full |
Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support |
title_fullStr |
Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support |
title_full_unstemmed |
Biochemical and physical characterization of immobilized Candida rugosa lipase on metal oxide hybrid support |
title_sort |
biochemical and physical characterization of immobilized candida rugosa lipase on metal oxide hybrid support |
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MDPI |
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2022 |
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http://eprints.utm.my/id/eprint/101344/1/NurIzyanWanAzelee2022_BiochemicalandPhysicalCharacterizationofImmobilizedCandidaRugosa.pdf http://eprints.utm.my/id/eprint/101344/ http://dx.doi.org/10.3390/catal12080854 |
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1769842040920604672 |