Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri
This study reports on a novel technique to enhance the high cell mass and viable cell counts of the heterofermentative probiotic strain, Limosilactobacillus reuteri. This is the first report on the cultivation of L. reuteri, which was incorporated with weak base anion-exchange resins to remove the a...
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my.utm.1039882024-01-09T00:45:09Z http://eprints.utm.my/103988/ Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri Selvamani, Shanmugaprakasham Ramli, Solleh Dailin, Daniel Joe Khairun Hani Natasya, Khairun Hani Natasya Varzakas, Theodoros Abomoelak, Bassam Dalia Sukmawati, Dalia Sukmawati Muktiningsih Nurjayadi, Muktiningsih Nurjayadi Liu, Siqing Gupta, Vijai Kumar El Enshasy, Hesham Ali TP Chemical technology This study reports on a novel technique to enhance the high cell mass and viable cell counts of the heterofermentative probiotic strain, Limosilactobacillus reuteri. This is the first report on the cultivation of L. reuteri, which was incorporated with weak base anion-exchange resins to remove the accumulating lactic acid in the fermentation broth. Two anion-exchange resins—Amberlite IRA 67 and IRA 96—were found to have a high adsorption capacity with lactic acid. Batch fermentation and fed-batch cultivation were further analyzed using IRA 67 resins, as this application resulted in a higher maximum number of viable cells. The in situ application of anion-exchange resins was found to create shear stress, and thus, it does not promote growth of L. reuteri, therefore, an external and integrated resin column system was proposed. The viable cell count from batch fermentation, when incorporated with the integrated resin column, was improved by 71 times (3.89 × 1011 ± 0.07 CFU mL-1) compared with control batch fermentation (5.35 × 109 ± 0.32 CFU mL-1), without the addition of resins. The growth improvement was achieved due to the high adsorption rate of lactic acid, which was recorded by the integrated IRA 67 resin system, and coupled with the stirred tank bioreactor batch fermentation process. MDPI 2022 Article PeerReviewed application/pdf en http://eprints.utm.my/103988/1/DanielJoeDailin2022_ExtractiveFermentationasANovelStrategy.pdf Selvamani, Shanmugaprakasham and Ramli, Solleh and Dailin, Daniel Joe and Khairun Hani Natasya, Khairun Hani Natasya and Varzakas, Theodoros and Abomoelak, Bassam and Dalia Sukmawati, Dalia Sukmawati and Muktiningsih Nurjayadi, Muktiningsih Nurjayadi and Liu, Siqing and Gupta, Vijai Kumar and El Enshasy, Hesham Ali (2022) Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri. Fermentation, 8 (10). pp. 1-15. ISSN 2311-5637 http://dx.doi.org/10.3390/fermentation8100527 DOI : 10.3390/fermentation8100527 |
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TP Chemical technology Selvamani, Shanmugaprakasham Ramli, Solleh Dailin, Daniel Joe Khairun Hani Natasya, Khairun Hani Natasya Varzakas, Theodoros Abomoelak, Bassam Dalia Sukmawati, Dalia Sukmawati Muktiningsih Nurjayadi, Muktiningsih Nurjayadi Liu, Siqing Gupta, Vijai Kumar El Enshasy, Hesham Ali Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri |
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This study reports on a novel technique to enhance the high cell mass and viable cell counts of the heterofermentative probiotic strain, Limosilactobacillus reuteri. This is the first report on the cultivation of L. reuteri, which was incorporated with weak base anion-exchange resins to remove the accumulating lactic acid in the fermentation broth. Two anion-exchange resins—Amberlite IRA 67 and IRA 96—were found to have a high adsorption capacity with lactic acid. Batch fermentation and fed-batch cultivation were further analyzed using IRA 67 resins, as this application resulted in a higher maximum number of viable cells. The in situ application of anion-exchange resins was found to create shear stress, and thus, it does not promote growth of L. reuteri, therefore, an external and integrated resin column system was proposed. The viable cell count from batch fermentation, when incorporated with the integrated resin column, was improved by 71 times (3.89 × 1011 ± 0.07 CFU mL-1) compared with control batch fermentation (5.35 × 109 ± 0.32 CFU mL-1), without the addition of resins. The growth improvement was achieved due to the high adsorption rate of lactic acid, which was recorded by the integrated IRA 67 resin system, and coupled with the stirred tank bioreactor batch fermentation process. |
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Article |
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Selvamani, Shanmugaprakasham Ramli, Solleh Dailin, Daniel Joe Khairun Hani Natasya, Khairun Hani Natasya Varzakas, Theodoros Abomoelak, Bassam Dalia Sukmawati, Dalia Sukmawati Muktiningsih Nurjayadi, Muktiningsih Nurjayadi Liu, Siqing Gupta, Vijai Kumar El Enshasy, Hesham Ali |
author_facet |
Selvamani, Shanmugaprakasham Ramli, Solleh Dailin, Daniel Joe Khairun Hani Natasya, Khairun Hani Natasya Varzakas, Theodoros Abomoelak, Bassam Dalia Sukmawati, Dalia Sukmawati Muktiningsih Nurjayadi, Muktiningsih Nurjayadi Liu, Siqing Gupta, Vijai Kumar El Enshasy, Hesham Ali |
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Selvamani, Shanmugaprakasham |
title |
Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri |
title_short |
Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri |
title_full |
Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri |
title_fullStr |
Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri |
title_full_unstemmed |
Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri |
title_sort |
extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain limosilactobacillus reuteri |
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MDPI |
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2022 |
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http://eprints.utm.my/103988/1/DanielJoeDailin2022_ExtractiveFermentationasANovelStrategy.pdf http://eprints.utm.my/103988/ http://dx.doi.org/10.3390/fermentation8100527 |
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