Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology

• Main Authors: Dahari, Rima Fatira, Misson, Mailin, Ongkudon, Clarence M., Saallah, Suryani, Asran, Aliyah Madihah, Dailin, Daniel Joe, Chuah, Lai Fatt, El Enshasy, Hesham Ali
• Format: Article
• Language: English
• Published: Malaysian Society for Microbiology 2023
• Subjects: Q Science (General)
• Online Access: http://eprints.utm.my/105430/1/DanielJoeDailin2023_EnhancingStabilityandActivityofBGalactosidase.pdf; http://eprints.utm.my/105430/; http://dx.doi.org/10.21161/MJM.230023
• Institution: Universiti Teknologi Malaysia

Aims: This study investigates the potential of porous polymethacrylate monoliths as enzyme support materials for large-scale enzyme commercialization. Methodology and results: It focuses on their preparation and various immobilization techniques, such as adsorption, covalent-binding and cross-linkin...