Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials.
The demand for advanced wound care products is rapidly increasing nowadays. In this study, gellan gum/collagen (GG/C) hydrogel films containing gatifloxacin (GAT) were developed to investigate their properties as wound dressing materials. ATR-FTIR, swelling, water content, water vapor transmission r...
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my.utm.1054702024-05-02T02:53:25Z http://eprints.utm.my/105470/ Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. Abu Bakar, Aznatul Jannah Mohd. Azam, Nurul Shahirah Sevakumaran, Vigneswari Wan Ismail, Wan Iryani Razali, Mohd. Hasmizam Abdul Razak, Saiful Izwan QD Chemistry QR180 Immunology The demand for advanced wound care products is rapidly increasing nowadays. In this study, gellan gum/collagen (GG/C) hydrogel films containing gatifloxacin (GAT) were developed to investigate their properties as wound dressing materials. ATR-FTIR, swelling, water content, water vapor transmission rate (WVTR), and thermal properties were investigated. The mechanical properties of the materials were tested in dry and wet conditions to understand the performance of the materials after exposure to wound exudate. Drug release by Franz diffusion was measured with all samples showing 100 % cumulative drug release after 40 min. Strong antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis were observed for Gram-positive bacteria, while Escherichia coli and Pseudomonas aeruginosa were observed for Gram-negative bacteria. The in-vivo cytotoxicity of GG/C-GAT was assessed by wound contraction in rats, which was 95 % for GG/C-GAT01. Hematoxylin and eosin and Masson's trichrome staining revealed the appearance of fresh full epidermis and granulation tissue, indicating that all wounds had passed through the proliferation phase. The results demonstrate the promising properties of the materials to be used as dressing materials. Elsevier B.V. 2023-08-01 Article PeerReviewed Abu Bakar, Aznatul Jannah and Mohd. Azam, Nurul Shahirah and Sevakumaran, Vigneswari and Wan Ismail, Wan Iryani and Razali, Mohd. Hasmizam and Abdul Razak, Saiful Izwan (2023) Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. International Journal of Biological Macromolecules, 245 (125494). NA-NA. ISSN 0141-8130 http://dx.doi.org/10.1016/j.ijbiomac.2023.125494 DOI: 10.1016/j.ijbiomac.2023.125494 |
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QD Chemistry QR180 Immunology Abu Bakar, Aznatul Jannah Mohd. Azam, Nurul Shahirah Sevakumaran, Vigneswari Wan Ismail, Wan Iryani Razali, Mohd. Hasmizam Abdul Razak, Saiful Izwan Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. |
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The demand for advanced wound care products is rapidly increasing nowadays. In this study, gellan gum/collagen (GG/C) hydrogel films containing gatifloxacin (GAT) were developed to investigate their properties as wound dressing materials. ATR-FTIR, swelling, water content, water vapor transmission rate (WVTR), and thermal properties were investigated. The mechanical properties of the materials were tested in dry and wet conditions to understand the performance of the materials after exposure to wound exudate. Drug release by Franz diffusion was measured with all samples showing 100 % cumulative drug release after 40 min. Strong antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis were observed for Gram-positive bacteria, while Escherichia coli and Pseudomonas aeruginosa were observed for Gram-negative bacteria. The in-vivo cytotoxicity of GG/C-GAT was assessed by wound contraction in rats, which was 95 % for GG/C-GAT01. Hematoxylin and eosin and Masson's trichrome staining revealed the appearance of fresh full epidermis and granulation tissue, indicating that all wounds had passed through the proliferation phase. The results demonstrate the promising properties of the materials to be used as dressing materials. |
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Article |
author |
Abu Bakar, Aznatul Jannah Mohd. Azam, Nurul Shahirah Sevakumaran, Vigneswari Wan Ismail, Wan Iryani Razali, Mohd. Hasmizam Abdul Razak, Saiful Izwan |
author_facet |
Abu Bakar, Aznatul Jannah Mohd. Azam, Nurul Shahirah Sevakumaran, Vigneswari Wan Ismail, Wan Iryani Razali, Mohd. Hasmizam Abdul Razak, Saiful Izwan |
author_sort |
Abu Bakar, Aznatul Jannah |
title |
Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. |
title_short |
Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. |
title_full |
Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. |
title_fullStr |
Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. |
title_full_unstemmed |
Effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. |
title_sort |
effectiveness of collagen and gatifloxacin in improving the healing and antibacterial activities of gellan gum hydrogel films as dressing materials. |
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Elsevier B.V. |
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2023 |
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http://eprints.utm.my/105470/ http://dx.doi.org/10.1016/j.ijbiomac.2023.125494 |
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1800082623316361216 |