Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli

Locally isolated fungi are screened for xylanase activity via Remazol Brilliant Blue-xylan agar plate for hydrolysis of 1,4-beta-D-xylosidic linkages and dinitrosalicylic acid (DNS) method for release of xylose. Two of the fungi which showed the highest xylanase activity is identified as Aspergillus...

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Main Author: Md. Illias, Rosli
Format: Conference or Workshop Item
Published: 2009
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Online Access:http://eprints.utm.my/id/eprint/14979/
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spelling my.utm.149792020-07-07T04:59:38Z http://eprints.utm.my/id/eprint/14979/ Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli Md. Illias, Rosli QD Chemistry QK Botany Locally isolated fungi are screened for xylanase activity via Remazol Brilliant Blue-xylan agar plate for hydrolysis of 1,4-beta-D-xylosidic linkages and dinitrosalicylic acid (DNS) method for release of xylose. Two of the fungi which showed the highest xylanase activity is identified as Aspergillus fumigatus RT-1, with activity 8.1 U/ml, followed by Aspergillus fumigatus RT-2, with activity 7.8 U/mL. Family 11 xylanase consensus fragments are amplified from genomic DNA by PCR using consensus degenerate primers that exhibited the conserved motifs within fungal family 11 xylanase genes. Two full-length xylanase genes, named xlnA and xynG1 are obtained by PCR and RT-PCR using specific primers designed based on the sequences in the database. The full-length xlnA contained 739 bp, including one intron of 52 bp while the full-length xynG1 contained 712 bp, with one intron of 46 bp. The mature peptide of xynG1 is cloned into pET-21a and pET-22b and expressed in Escherichia coli BL21 (DE3). 2009 Conference or Workshop Item PeerReviewed Md. Illias, Rosli (2009) Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli. In: 2nd International Conference & Workshops on Basic & Applied Sciences and Regional Annual Fundamental Science Seminar 2009 (ICORAFSS 2009), 2009, The ZON Regency Hotel, Johor. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:99635
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
QK Botany
spellingShingle QD Chemistry
QK Botany
Md. Illias, Rosli
Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli
description Locally isolated fungi are screened for xylanase activity via Remazol Brilliant Blue-xylan agar plate for hydrolysis of 1,4-beta-D-xylosidic linkages and dinitrosalicylic acid (DNS) method for release of xylose. Two of the fungi which showed the highest xylanase activity is identified as Aspergillus fumigatus RT-1, with activity 8.1 U/ml, followed by Aspergillus fumigatus RT-2, with activity 7.8 U/mL. Family 11 xylanase consensus fragments are amplified from genomic DNA by PCR using consensus degenerate primers that exhibited the conserved motifs within fungal family 11 xylanase genes. Two full-length xylanase genes, named xlnA and xynG1 are obtained by PCR and RT-PCR using specific primers designed based on the sequences in the database. The full-length xlnA contained 739 bp, including one intron of 52 bp while the full-length xynG1 contained 712 bp, with one intron of 46 bp. The mature peptide of xynG1 is cloned into pET-21a and pET-22b and expressed in Escherichia coli BL21 (DE3).
format Conference or Workshop Item
author Md. Illias, Rosli
author_facet Md. Illias, Rosli
author_sort Md. Illias, Rosli
title Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli
title_short Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli
title_full Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli
title_fullStr Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli
title_full_unstemmed Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli
title_sort cloning of family 11 xylanase, xyng1 from locally isolated aspergillus fumigatus rt-1 & its expression in escherichia coli
publishDate 2009
url http://eprints.utm.my/id/eprint/14979/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:99635
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