Molecular cloning of a cyclodextrin glucanotransferase gene from alkalophilic Bacillus sp. TS1-1 and characterization of the recombinant enzyme

A cyclodextrin glucanotransferase (CGTase) gene from Bacillus sp. TS1-1 was isolated and cloned into Escherichia coli. Starting from TTG codon, there was an open reading frame composed of 2163 bp (721 amino acids). The NH2 terminal position encoded a 46-amino acid of a signal peptide and followed by...

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Bibliographic Details
Main Authors: Rahman, Kamalesh, Mohd Illias, Rosli, Hassan, Osman, Nik Mahmood, Nik Azmi, Abdul Rashid, Noor Aini
Format: Article
Language:English
Published: Elsevier Inc. 2006
Subjects:
Online Access:http://eprints.utm.my/id/eprint/168/1/EMTNAR2006.pdf
http://eprints.utm.my/id/eprint/168/
http://dx.doi.org/10.1016/j.enzmictec.2005.09.014
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Institution: Universiti Teknologi Malaysia
Language: English