Optimization and scaling up of penicillin acylase production process by escherichia coli

• Main Authors: EI Enshasy, Hesham A., Mohy EI Din, Mohamed S., El Sayed, Mohamed, El Sayed, Salah, Haroun, Bakry M., Hassan, Elsayed A.
• Format: Article
• Language: English
• Published: IDOSI Publications, 2009
• Subjects: T Technology (General); TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/21028/1/HeshamAEIEnshasy2009_OptimizationAndScalingUpOfPenicillinAcylase.pdf; http://eprints.utm.my/id/eprint/21028/
• Institution: Universiti Teknologi Malaysia

In the present study, the standard strain of Escherichia coli ATCC 11105 was used to develop an industrial process for penicillin G acylase (PAC) production. Among different media used, media composed of tryptone, yeast extract, sodium chloride and glucose yielded the highest volumetric PAC producti...