Enhanced secretory production of hemolysin-mediated cyclodextrin glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system

• Main Authors: Low, Kheng Oon, Mahadi, Nor Muhammad, Abdul Rahim, Raha, Rabu, Amir, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir, Md. Illias, Rosli
• Format: Article
• Published: Elsevier B.V. 2010
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/26167/; http://dx.doi.org/10.1016/j.jbiotec.2010.10.001
• Institution: Universiti Teknologi Malaysia

The hemolysin transport system was found to mediate the release of cyclodextringlucanotransferase (CGTase) into the extracellular medium when it was fused to the C-terminal 61 amino acids of HlyA (HlyAs61). To produce an improved-secretion variant, the hly components (hlyAs, hlyB and hlyD) were engi...