A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli

• Main Authors: Ismail, Noor Faizah, Hamdan, Salehhuddin, Mahadi, Nor Muhammad, Abdul Murad, Abdul Munir, Rabu, Amir, Abu Bakar, Farah Diba, Klappa, Peter, Md. Illias, Rosli
• Format: Article
• Published: Springer Science+Business Media B.V. 2011
• Subjects: QH301 Biology
• Online Access: http://eprints.utm.my/id/eprint/28580/; http://dx.doi.org/10.1007/s10529-011-0517-8
• Institution: Universiti Teknologi Malaysia

l-Asparaginase II signal peptide was used for the secretion of recombinant cyclodextrin glucanotransferase (CGTase) into the periplasmic space of E. coli. Despite its predominant localisation in the periplasm, CGTase activity was also detected in the extracellular medium, followed by cell lysis. Fiv...