An effective extracellular protein secretion by an ABC transporter system in Escherichia coli: statistical modeling and optimization of cyclodextrin glucanotransferase secretory production

• Main Authors: Low, Kheng Oon, Muhammad Mahadi, Nor, Abdul Rahim, Raha, Rabu, Amir, Abu Bakar, Farah Diba, Abd. Murad, Abd. Munir, Md. Illias, Rosli
• Format: Article
• Published: Springer International Publishing 2011
• Subjects: TA Engineering (General). Civil engineering (General); TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/28676/; http://dx.doi.org/10.1007/s10295-011-0949-0
• Institution: Universiti Teknologi Malaysia

Direct transport of recombinant protein from cytosol to extracellular medium offers great advantages, such as high specific activity and a simple purification step. This work presents an investigation on the potential of an ABC (ATP-binding cassette) transporter system, the hemolysin transport syste...