The influence of mixing on lysozyme renaturation during refolding in an oscillatory flow and a stirred-tank reactor

• Main Authors: Lee, Chew Tin, Buswell, A. Mark, Middleberg, P. J.
• Format: Article
• Language: English
• Published: 2002
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/3084/1/CES.pdf; http://eprints.utm.my/id/eprint/3084/
• Institution: Universiti Teknologi Malaysia

Protein refolding is a key unit operation in many processes that produce recombinant biopharmaceuticals using Escherichia coli. Yield in this step generally controls overall process yield, and at industrially relevant protein concentrations is limited by aggregation. While most refolding operations...