Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture

Medium is the most important item in cell culture. Each medium consists of two main parts which are a basal medium and serum. The main source of the serum is come from an animal blood. The serum from animal blood has many disadvantages such as viral contaminations. Moreover, serum is very expensive....

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Main Author: Kazemzadeh Farizhandi, Amir Abbas
Format: Thesis
Language:English
Published: 2012
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Online Access:http://eprints.utm.my/id/eprint/32433/1/AmirAbbasKazemzadehFarizhandiMFCE2012.pdf
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Institution: Universiti Teknologi Malaysia
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spelling my.utm.324332017-08-09T08:22:31Z http://eprints.utm.my/id/eprint/32433/ Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture Kazemzadeh Farizhandi, Amir Abbas Q Science (General) Medium is the most important item in cell culture. Each medium consists of two main parts which are a basal medium and serum. The main source of the serum is come from an animal blood. The serum from animal blood has many disadvantages such as viral contaminations. Moreover, serum is very expensive. Serum-free media is the best alternative in solving this problem. Unfortunately, serum-free medium is not sufficient for the cell growth. Thus, some supplements must be added to serum-free medium. One of the most important sources of supplements is from plants which could reduce the cost. In this study different plant protein hydrolysates from soy, sesame, Aloe vera, rice and wheat have been tested against Human Skin Fibroblast (HSF) 1184. All protein hydrolysates were prepared through enzymatic hydrolysis using commercial enzymes of non-animal origin. These hydrolysates were characterized according to their solubility and peptide size. Different growth behaviours of HSF 1184 cells were observed when these hydrolysates were added in DMEM with and without Fetal Bovine Serum (FBS). Hydrolysates from exopeptidase enzymes such as Flavourzyme gave negative effect on HSF 1184 cell culture, while hydrolysates from endopeptidase enzymes were supplementary for HSF 1184 cell culture. Since plant proteins do not have all the necessary amino acids for HSF 1184 cell culture growth, they cannot be solely substituted with FBS. Depending on the enzyme used, the supplementation with hydrolysates corresponding to a high degree of hydrolysis and composition of peptides with small molecular size, led to different maximal cell density. This indicates the importance of enzyme specificity and consequently the nature of the released peptides. In conclusion, the best plant protein hydrolysates for supplementation into the complete medium for HSF 1184 growth were soy and Aloe vera which were hydrolysed by Alcalase. 2012 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/32433/1/AmirAbbasKazemzadehFarizhandiMFCE2012.pdf Kazemzadeh Farizhandi, Amir Abbas (2012) Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture. Masters thesis, Universiti Teknologi Malaysia, Faculty of Chemical Engineering dan Kejuruteraan Sumber Asli. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:70143?site_name=Restricted Repository
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Kazemzadeh Farizhandi, Amir Abbas
Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
description Medium is the most important item in cell culture. Each medium consists of two main parts which are a basal medium and serum. The main source of the serum is come from an animal blood. The serum from animal blood has many disadvantages such as viral contaminations. Moreover, serum is very expensive. Serum-free media is the best alternative in solving this problem. Unfortunately, serum-free medium is not sufficient for the cell growth. Thus, some supplements must be added to serum-free medium. One of the most important sources of supplements is from plants which could reduce the cost. In this study different plant protein hydrolysates from soy, sesame, Aloe vera, rice and wheat have been tested against Human Skin Fibroblast (HSF) 1184. All protein hydrolysates were prepared through enzymatic hydrolysis using commercial enzymes of non-animal origin. These hydrolysates were characterized according to their solubility and peptide size. Different growth behaviours of HSF 1184 cells were observed when these hydrolysates were added in DMEM with and without Fetal Bovine Serum (FBS). Hydrolysates from exopeptidase enzymes such as Flavourzyme gave negative effect on HSF 1184 cell culture, while hydrolysates from endopeptidase enzymes were supplementary for HSF 1184 cell culture. Since plant proteins do not have all the necessary amino acids for HSF 1184 cell culture growth, they cannot be solely substituted with FBS. Depending on the enzyme used, the supplementation with hydrolysates corresponding to a high degree of hydrolysis and composition of peptides with small molecular size, led to different maximal cell density. This indicates the importance of enzyme specificity and consequently the nature of the released peptides. In conclusion, the best plant protein hydrolysates for supplementation into the complete medium for HSF 1184 growth were soy and Aloe vera which were hydrolysed by Alcalase.
format Thesis
author Kazemzadeh Farizhandi, Amir Abbas
author_facet Kazemzadeh Farizhandi, Amir Abbas
author_sort Kazemzadeh Farizhandi, Amir Abbas
title Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_short Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_full Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_fullStr Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_full_unstemmed Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_sort plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
publishDate 2012
url http://eprints.utm.my/id/eprint/32433/1/AmirAbbasKazemzadehFarizhandiMFCE2012.pdf
http://eprints.utm.my/id/eprint/32433/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:70143?site_name=Restricted Repository
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