Chemical compositions and antibacterial activity of the leaf and stem oils of piper porphyrophyllum (Lindl.) Ne Br.E

• Main Authors: Wan Salleh, Wan Mohd. Nuzul Hakimi, Ahmad, Farediah, Mohd. Sirat, Hasnah, Khong, Heng Yen
• Format: Article
• Published: Leibniz Research Centre for Working Environment and Human Factors 2012
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/33003/; http://www.excli.de/vol11/Ahmad07_2012/Ahmad_02072012_proof.pdf
• Institution: Universiti Teknologi Malaysia

The essential oils obtained by hydrodistillation from the fresh leaf and stem of Piper porphy-rophyllum N.E. Br. were analyzed by GC and GC/MS. Thirty four constituents were identified in the leaf oil, while thirty eight constitue nts were identified in the stems oil. The most abundant components in the leaf oil included bicyclogermacrene (14.7%), α-copaene (13.2%) and β-phellandrene (9.5%) while sabinene (15.5%), bicyclogermacrene (12.3%) and α-copaene (8.1%) were the main constituents in the stem oil. The evaluation of antibact e-rial activity by using micro-dilution method revealed that both oils were moderately active against all the Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Pseudomonas aeruginosa, Pseudomonas putida and Escherichia coli) with minimum inhibitory concentration (MIC) values in the range 125-1000 μg/ml.