Construction of a strep-tag II mutant maltose binding protein for reagentless fluorescence sensing
Maltose binding protein (MBP) changes its conformational structure upon its ligand binding.This molecular recognition element that transduces a ligand-binding event into a physical one make MBP an ideal candidate for reagentless fluorescence sensing. MBP gene, (malE) was amplified from a pMaL-C4x pl...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/34593/1/SitiHalimahHasmoniMFBB2012.pdf http://eprints.utm.my/id/eprint/34593/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69748?site_name=Restricted Repository |
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| Institution: | Universiti Teknologi Malaysia |
| Language: | English |