Precipitation of cellulase and xylanase for cross-linked enzyme aggregates

Cross-linked enzyme aggregate is a promising strategy among other enzyme immobilization technologies such as solid matrix linking and gel entrapping. Despite of having the advantage of being reused, cross-linked enzyme aggregate (CLEA) also offers greater stability during operation and storage. Prep...

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Main Authors: Sulaiman, Nurul Jannah, A. Rahman, Roshanida, Ngadi, Norzita
Format: Conference or Workshop Item
Published: 2013
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Online Access:http://eprints.utm.my/id/eprint/39600/
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spelling my.utm.396002017-09-13T07:00:44Z http://eprints.utm.my/id/eprint/39600/ Precipitation of cellulase and xylanase for cross-linked enzyme aggregates Sulaiman, Nurul Jannah A. Rahman, Roshanida Ngadi, Norzita QD Chemistry Cross-linked enzyme aggregate is a promising strategy among other enzyme immobilization technologies such as solid matrix linking and gel entrapping. Despite of having the advantage of being reused, cross-linked enzyme aggregate (CLEA) also offers greater stability during operation and storage. Preparation of CLEA involves two steps which are precipitation and cross-linking of the enzymes. The purpose of this study is to find the best precipitant for cross-linked enzyme aggregate of cellulase and xylanase. The tested precipitants were acetone, ammonium sulphate, dimethoxyethane (DME), n-propanol, polyethyleneglycol (PEG), and tert-butanol. The enzymes were precipitated and cross-linked using glutaraldehyde. The enzyme activities were determined through DNS method and the relative activities for resulted CLEA were compared. It was found that PEG was the best precipitant for CLEA-cellulase while DME, ammonium sulphate and tert-butanol contributed the highest activity retention for CLEA-cellulase-xylanase under cellulase and xylanase assay, and CLEA-xylanase, respectively. 2013 Conference or Workshop Item PeerReviewed Sulaiman, Nurul Jannah and A. Rahman, Roshanida and Ngadi, Norzita (2013) Precipitation of cellulase and xylanase for cross-linked enzyme aggregates. In: International Conference on separation Technology, Icost, 2013.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Sulaiman, Nurul Jannah
A. Rahman, Roshanida
Ngadi, Norzita
Precipitation of cellulase and xylanase for cross-linked enzyme aggregates
description Cross-linked enzyme aggregate is a promising strategy among other enzyme immobilization technologies such as solid matrix linking and gel entrapping. Despite of having the advantage of being reused, cross-linked enzyme aggregate (CLEA) also offers greater stability during operation and storage. Preparation of CLEA involves two steps which are precipitation and cross-linking of the enzymes. The purpose of this study is to find the best precipitant for cross-linked enzyme aggregate of cellulase and xylanase. The tested precipitants were acetone, ammonium sulphate, dimethoxyethane (DME), n-propanol, polyethyleneglycol (PEG), and tert-butanol. The enzymes were precipitated and cross-linked using glutaraldehyde. The enzyme activities were determined through DNS method and the relative activities for resulted CLEA were compared. It was found that PEG was the best precipitant for CLEA-cellulase while DME, ammonium sulphate and tert-butanol contributed the highest activity retention for CLEA-cellulase-xylanase under cellulase and xylanase assay, and CLEA-xylanase, respectively.
format Conference or Workshop Item
author Sulaiman, Nurul Jannah
A. Rahman, Roshanida
Ngadi, Norzita
author_facet Sulaiman, Nurul Jannah
A. Rahman, Roshanida
Ngadi, Norzita
author_sort Sulaiman, Nurul Jannah
title Precipitation of cellulase and xylanase for cross-linked enzyme aggregates
title_short Precipitation of cellulase and xylanase for cross-linked enzyme aggregates
title_full Precipitation of cellulase and xylanase for cross-linked enzyme aggregates
title_fullStr Precipitation of cellulase and xylanase for cross-linked enzyme aggregates
title_full_unstemmed Precipitation of cellulase and xylanase for cross-linked enzyme aggregates
title_sort precipitation of cellulase and xylanase for cross-linked enzyme aggregates
publishDate 2013
url http://eprints.utm.my/id/eprint/39600/
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