Enantioseparation of enilconazole using cyclodextrin-modified MEKC
Enantiomeric separation of enilconazole has been successfully achieved using cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) method with HP-γ-CD as chiral selector. Two parameters which were HP-γ-CD concentration and SDS concentration have been optimized by investigating their...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit UTM Press
2011
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/39922/1/DadanHermawan2011_EnantioseparationofEnilconazoleusingCyclodextrin.pdf http://eprints.utm.my/id/eprint/39922/ https://dx.doi.org/10.11113/mjfas.v7n1.241 |
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| Institution: | Universiti Teknologi Malaysia |
| Language: | English |
| Summary: | Enantiomeric separation of enilconazole has been successfully achieved using cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) method with HP-γ-CD as chiral selector. Two parameters which were HP-γ-CD concentration and SDS concentration have been optimized by investigating their effects on resolution and analysis time. Enantioresolution of enilconazole was achieved by the optimized CD-MEKC system containing 40 mM HP-γ-CD, 40mM SDS and 20mM phosphate buffer (pH8) with resolution (Rs=1.3) and analysis time within 9min. Good repeatabilities were obtained in term of RSD% (n=3) for migration time, peak area and peak height ranging from 0.07 to 6.1%. The optimized CD-MEKC method was a simple, less solvent used, and able to perform enantiomeric separation of enilconazole with a good resolution and relatively short analysis time. |
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