Investigation of 1h NMR profile of vegetarian human urine using anova-based multi-factor analysis

• Main Authors: Dong, J. Y., Deng, L. L., Cheng, K. K., Griffin, J. L., Chen, Z.
• Format: Article
• Published: Jilin Daxue,Jilin University 2011
• Subjects: QP Physiology
• Online Access: http://eprints.utm.my/id/eprint/45006/
• Institution: Universiti Teknologi Malaysia

In this study,a technique that combined both analysis of variance(ANOVA) and partial least squares-discriminant analysis(PLS-DA) was used to compare the urine 1H NMR spectra of healthy people from a vegetarian and omnivorous population.In ANOVA/PLS-DA,the variation in data was first decomposed into different variance components that each contains a single source of variation.Each of the resulting variance components was then analyzed using PLS-DA.The experimental results showed that ANOVA/PLS-DA is efficient in disentangling the effect of diet and gender on the metabolic profile,and the method could be used to extract biologically relevant information for result interpretation.