Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis

• Main Authors: Baradaran, Ali, Sieo, Chin Chin, Foo, Hooi Ling, Md. Illias, Rosli, Yusoff, Khatijah, Abdul Rahim, Raha
• Format: Article
• Published: Springer Science+Business Media Dordrecht 2013
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/49249/; https://doi.org/10.1007/s10529-012-1059-4
• Institution: Universiti Teknologi Malaysia

Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (G...