Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127

The aim of this study is to study the effect of various concentrations of 2,4-D on embryogenic callus induction of indica rice MR123 cultivar and MR127 cultivar using mature seeds. Optimal media for induction of callus of both cultivars was MS basal media supplemented with 30g/L sucrose, 500mg/L glu...

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Main Authors: Illyas Ahmad, Fauziah, Johan, Nur Shafiqoh, Wagiran, Alina
Format: Article
Language:English
Published: Penerbit UTM 2013
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Online Access:http://eprints.utm.my/id/eprint/50026/1/FauziahIllyasAhmad2013_Effectof2%2C4-donembryogenic.pdf
http://eprints.utm.my/id/eprint/50026/
http://dx.doi.org/10.11113/jt.v64.2048
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Institution: Universiti Teknologi Malaysia
Language: English
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spelling my.utm.500262018-09-30T08:17:05Z http://eprints.utm.my/id/eprint/50026/ Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127 Illyas Ahmad, Fauziah Johan, Nur Shafiqoh Wagiran, Alina R Medicine (General) The aim of this study is to study the effect of various concentrations of 2,4-D on embryogenic callus induction of indica rice MR123 cultivar and MR127 cultivar using mature seeds. Optimal media for induction of callus of both cultivars was MS basal media supplemented with 30g/L sucrose, 500mg/L glutamine and 500mg/L proline supplemented with 2.5 mg/L 2,4-D. The highest percentage of callus induction was 70% and 76% for MR123 and MR127 respectively. Both cultivars produced an embryogenic callus from scutellum after a week in culture with white-yellowish in color. The viability tested using Evans blue show callus obtained was embryogenic. This simple protocol using staining method could be used for screening embryogenic callus before further experiments can be conducted Penerbit UTM 2013 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/50026/1/FauziahIllyasAhmad2013_Effectof2%2C4-donembryogenic.pdf Illyas Ahmad, Fauziah and Johan, Nur Shafiqoh and Wagiran, Alina (2013) Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127. Jurnal Teknologi (Sciences and Engineering), 64 (2). pp. 75-78. ISSN 0127-9696 http://dx.doi.org/10.11113/jt.v64.2048 DOI: 10.11113/jt.v64.2048
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Illyas Ahmad, Fauziah
Johan, Nur Shafiqoh
Wagiran, Alina
Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127
description The aim of this study is to study the effect of various concentrations of 2,4-D on embryogenic callus induction of indica rice MR123 cultivar and MR127 cultivar using mature seeds. Optimal media for induction of callus of both cultivars was MS basal media supplemented with 30g/L sucrose, 500mg/L glutamine and 500mg/L proline supplemented with 2.5 mg/L 2,4-D. The highest percentage of callus induction was 70% and 76% for MR123 and MR127 respectively. Both cultivars produced an embryogenic callus from scutellum after a week in culture with white-yellowish in color. The viability tested using Evans blue show callus obtained was embryogenic. This simple protocol using staining method could be used for screening embryogenic callus before further experiments can be conducted
format Article
author Illyas Ahmad, Fauziah
Johan, Nur Shafiqoh
Wagiran, Alina
author_facet Illyas Ahmad, Fauziah
Johan, Nur Shafiqoh
Wagiran, Alina
author_sort Illyas Ahmad, Fauziah
title Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127
title_short Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127
title_full Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127
title_fullStr Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127
title_full_unstemmed Effect of 2,4-d on embryogenic callus induction of Malaysian indica rice (oryza sativa l) cultivars mr123 and mr127
title_sort effect of 2,4-d on embryogenic callus induction of malaysian indica rice (oryza sativa l) cultivars mr123 and mr127
publisher Penerbit UTM
publishDate 2013
url http://eprints.utm.my/id/eprint/50026/1/FauziahIllyasAhmad2013_Effectof2%2C4-donembryogenic.pdf
http://eprints.utm.my/id/eprint/50026/
http://dx.doi.org/10.11113/jt.v64.2048
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