Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering

Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have developed a novel sonication decellularization system to prepare decellularized bio-scaffolds in a short treatment time. The aim of the study is to investigate sonication decellularization conditio...

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Main Authors: Azhim, Azran, Ono, T., Fukui, Yasuhiro, Morimoto, Yuji, Furukawa, K., Ushida, T.
Format: Conference or Workshop Item
Published: 2013
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Online Access:http://eprints.utm.my/id/eprint/51258/
http://ieeexplore.ieee.org/document/6611157/
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Institution: Universiti Teknologi Malaysia
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spelling my.utm.512582017-09-03T11:38:36Z http://eprints.utm.my/id/eprint/51258/ Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering Azhim, Azran Ono, T. Fukui, Yasuhiro Morimoto, Yuji Furukawa, K. Ushida, T. RM Therapeutics. Pharmacology Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have developed a novel sonication decellularization system to prepare decellularized bio-scaffolds in a short treatment time. The aim of the study is to investigate sonication decellularization condition that completely decellularize meniscus can be changed as well as to maintain the biomechanical parameters of scaffolds. The meniscus samples were decellularized using sonication treatment. The treated samples were evaluated histologically by EVG for cell removal, picrosirius red for content of collagen type I and III, and safranin-O/fast green staining for content of glycosaminoglycan, and SEM for observation of scaffold surface. Indentation apparatus was used to analyze the unconfined deformation under load of native and decellularized menisci. The load parameters which are stiffness, compression and residual force were not significantly different compare with native and sonicated scaffolds. However, the content of extracellular matrix and its fiber alignment changed significantly due to sonication treatment as observed by SEM and safranin-O/fast green staining, respectively. The removal of immunogenic cell components by sonication decellularization as well as maintain its biomechanical strength of decellularized scaffolds, so that it has potential to use as an implant material for tissue engineering of menisci. 2013 Conference or Workshop Item PeerReviewed Azhim, Azran and Ono, T. and Fukui, Yasuhiro and Morimoto, Yuji and Furukawa, K. and Ushida, T. (2013) Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering. In: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2013, 3rd-7th July 2013, Osaka, Japan. http://ieeexplore.ieee.org/document/6611157/
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic RM Therapeutics. Pharmacology
spellingShingle RM Therapeutics. Pharmacology
Azhim, Azran
Ono, T.
Fukui, Yasuhiro
Morimoto, Yuji
Furukawa, K.
Ushida, T.
Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
description Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have developed a novel sonication decellularization system to prepare decellularized bio-scaffolds in a short treatment time. The aim of the study is to investigate sonication decellularization condition that completely decellularize meniscus can be changed as well as to maintain the biomechanical parameters of scaffolds. The meniscus samples were decellularized using sonication treatment. The treated samples were evaluated histologically by EVG for cell removal, picrosirius red for content of collagen type I and III, and safranin-O/fast green staining for content of glycosaminoglycan, and SEM for observation of scaffold surface. Indentation apparatus was used to analyze the unconfined deformation under load of native and decellularized menisci. The load parameters which are stiffness, compression and residual force were not significantly different compare with native and sonicated scaffolds. However, the content of extracellular matrix and its fiber alignment changed significantly due to sonication treatment as observed by SEM and safranin-O/fast green staining, respectively. The removal of immunogenic cell components by sonication decellularization as well as maintain its biomechanical strength of decellularized scaffolds, so that it has potential to use as an implant material for tissue engineering of menisci.
format Conference or Workshop Item
author Azhim, Azran
Ono, T.
Fukui, Yasuhiro
Morimoto, Yuji
Furukawa, K.
Ushida, T.
author_facet Azhim, Azran
Ono, T.
Fukui, Yasuhiro
Morimoto, Yuji
Furukawa, K.
Ushida, T.
author_sort Azhim, Azran
title Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_short Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_full Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_fullStr Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_full_unstemmed Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_sort preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
publishDate 2013
url http://eprints.utm.my/id/eprint/51258/
http://ieeexplore.ieee.org/document/6611157/
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