Bio-active constituents of rotenoids resin extracted from Derris elliptica roots:comparison between local plant extract and SAPHYR (France)cube resin

• Main Authors: Zubairi, Saiful Irwan, Sarmidi, Mohamad Roji, Abdul Aziz, Ramlan
• Format: Article
• Published: American-Eurasian Network for Scientific Information 2014
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/51995/; http://www.aensiweb.com
• Institution: Universiti Teknologi Malaysia

It is well known now that some plant species represent an efficient factory of chemicals, which are manufactured and used as bio-weapons against pest attacks. Extensive work has been done since the last few decades on these potentially useful compounds. During the last few decades a growing interest was directed towards a safer agricultural production such as free residual toxicity hazards to human beings and to the environment. For instance, plant-based extracts biocides possess a greater advantage compared with the chemical ones. Their efficacies are also acceptable. In this work, the aim is to standardize and determine the bio-active constituents extracted from local plant of Derris species (Derris elliptica) by using an internal standard method of reversed-phase high performance liquid chromatography (RP-HPLC) system. The raw plants were collected from Kota Johor Lama, Malaysia and sorted to collect the roots and stems. Rotenoids resin from the roots and stems were extracted by using a normal soaking extraction (NSE) method at 30 ± 2 °C. A solvent of acetone with an optimum solvent-to-solid ratio of 10 ml/g was utilized in the extraction process. The extraction was carried out for 24 hrs and the extract was filtered to remove any fine debris prior to the RP-HPLC analysis. The commercially available rotenoids resin cube of SAPHYR (France) was analyzed to compare and verify the bio-active constituents available with the extract of local plant species. The RP-HPLC analysis results showed the elicited distinguishable patterns of the bio-active constituents between the extract of Derris elliptica and commercial grade of rotenoids cube resin. This extract has a great potential to be used as insecticidal products. However, the rotenone content is still low as compared to the commercial grade rotenoids resin. For that reason, a plant tissue culture is needed to produce a hybrid species between Derris elliptica and Amazonia species so that high yield of rotenone could be attained.