Development of HPLC fingerprint analysis of traditional diabetes herbal Jamu Diabetes plant materials

• Main Authors: Shams, Gholamhossein, Ismail, Hassan Fahmi, Teh, Liam Chee, Ali, M. Abbas, Abdul Majid, Fadzilah Adibah, Sarmidi, Mohamad Roji, Abdul Aziz, Ramlan
• Format: Article
• Language: English
• Published: Penerbit UTM 2014
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/52374/1/HassanFahmiIsmail2014_DevelopmentofHPLCfingerprint.pdf; http://eprints.utm.my/id/eprint/52374/; https://dx.doi.org/10.11113/jt.v68.1686
• Institution: Universiti Teknologi Malaysia

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. In the present study, high-performance liquid chromatography (HPLC) detection was developed for chromatographic fingerprint analysis of the extract from grounded plant materials of Jamu Diabetes (JD), a well known herbal formulation in Malaysia. The bioactive markers rosmarinic acid, curcumin, cinnamaldehyde and andrographolide were identified from the methanol/water (80:20) extract of JD materials. The data presented in this study showed that the developed method is simple, sensitive and specific for simultaneous determination of the indicated marker compounds either qualitatively or quantitatively, and may be used as a fingerprint profile for the standardization of extractives or herbal medicines from herbal formulation JD materials