Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes

Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using respons...

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Main Authors: Sathishkumar, Palanivel, Mythili, Arumugam, Hadibarata, Tony, Jayakumar, Rajarajeswaran, Kanthimathi, M. S., Thayumanavan, Palvannan, Ponraj, Mohanadoss, Salim, Mohd. Razman, Mohd. Yusoff, Abdull Rahim
Format: Article
Language:English
Published: The Royal Society of Chemistry 2014
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Online Access:http://eprints.utm.my/id/eprint/53295/1/PalanivelSathishkumar2014_Laccasemediateddiclofenactransformation.pdf
http://eprints.utm.my/id/eprint/53295/
http://dx.doi.org/10.1039/c3ra46014b
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Institution: Universiti Teknologi Malaysia
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spelling my.utm.532952018-07-25T07:55:25Z http://eprints.utm.my/id/eprint/53295/ Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes Sathishkumar, Palanivel Mythili, Arumugam Hadibarata, Tony Jayakumar, Rajarajeswaran Kanthimathi, M. S. Thayumanavan, Palvannan Ponraj, Mohanadoss Salim, Mohd. Razman Mohd. Yusoff, Abdull Rahim TA Engineering (General). Civil engineering (General) Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using response surface methodology (RSM) and cytotoxicity testing on mouse fibroblast 3T3-L1 preadipocytes. Three factors (laccase, syringaldehyde, reaction time) were used to optimize the diclofenac transformation. The optimum level of laccase, syringaldehyde and reaction time was found to be 1.91 U mL -1, 187 μg and 51 min for diclofenac transformation (20 mg L -1). The cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes showed that a maximum of 67.9% cell death occurred at 72 h treatment with diclofenac (200 μg mL-1), while the cells treated with laccase treated diclofenac (LTD) showed less toxicity on the cells. These findings can be addressed for the removal of diclofenac toxicity The Royal Society of Chemistry 2014 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/53295/1/PalanivelSathishkumar2014_Laccasemediateddiclofenactransformation.pdf Sathishkumar, Palanivel and Mythili, Arumugam and Hadibarata, Tony and Jayakumar, Rajarajeswaran and Kanthimathi, M. S. and Thayumanavan, Palvannan and Ponraj, Mohanadoss and Salim, Mohd. Razman and Mohd. Yusoff, Abdull Rahim (2014) Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes. RSC Advances, 4 (23). pp. 11689-11697. ISSN 2046-2069 http://dx.doi.org/10.1039/c3ra46014b DOI: 10.1039/c3ra46014b
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TA Engineering (General). Civil engineering (General)
spellingShingle TA Engineering (General). Civil engineering (General)
Sathishkumar, Palanivel
Mythili, Arumugam
Hadibarata, Tony
Jayakumar, Rajarajeswaran
Kanthimathi, M. S.
Thayumanavan, Palvannan
Ponraj, Mohanadoss
Salim, Mohd. Razman
Mohd. Yusoff, Abdull Rahim
Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes
description Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using response surface methodology (RSM) and cytotoxicity testing on mouse fibroblast 3T3-L1 preadipocytes. Three factors (laccase, syringaldehyde, reaction time) were used to optimize the diclofenac transformation. The optimum level of laccase, syringaldehyde and reaction time was found to be 1.91 U mL -1, 187 μg and 51 min for diclofenac transformation (20 mg L -1). The cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes showed that a maximum of 67.9% cell death occurred at 72 h treatment with diclofenac (200 μg mL-1), while the cells treated with laccase treated diclofenac (LTD) showed less toxicity on the cells. These findings can be addressed for the removal of diclofenac toxicity
format Article
author Sathishkumar, Palanivel
Mythili, Arumugam
Hadibarata, Tony
Jayakumar, Rajarajeswaran
Kanthimathi, M. S.
Thayumanavan, Palvannan
Ponraj, Mohanadoss
Salim, Mohd. Razman
Mohd. Yusoff, Abdull Rahim
author_facet Sathishkumar, Palanivel
Mythili, Arumugam
Hadibarata, Tony
Jayakumar, Rajarajeswaran
Kanthimathi, M. S.
Thayumanavan, Palvannan
Ponraj, Mohanadoss
Salim, Mohd. Razman
Mohd. Yusoff, Abdull Rahim
author_sort Sathishkumar, Palanivel
title Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes
title_short Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes
title_full Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes
title_fullStr Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes
title_full_unstemmed Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes
title_sort laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3t3-l1 preadipocytes
publisher The Royal Society of Chemistry
publishDate 2014
url http://eprints.utm.my/id/eprint/53295/1/PalanivelSathishkumar2014_Laccasemediateddiclofenactransformation.pdf
http://eprints.utm.my/id/eprint/53295/
http://dx.doi.org/10.1039/c3ra46014b
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