Acetylcholinesterase activity of crude extracts and flavonoids from artocarpus anisophyllus miq. And artocarpus lowii king.

• Main Authors: Jamil, Shajarahtunnur, Abdul Lathiff, Siti Mariam, Abdullah, Siti Awanis
• Format: Article
• Language: English
• Published: Universiti Pendidikan Sultan Idris 2022
• Subjects: Q Science (General)
• Online Access: http://eprints.utm.my/56137/1/SitiAwanisAbdullahSitiMariamAbdulLathiffShajarahtunnurJamil2022_AcetylcholinesteraseActivityofCrudeExtracts.pdf; http://eprints.utm.my/56137/; https://ejournal.upsi.edu.my/index.php/JSML/article/view/7009
• Institution: Universiti Teknologi Malaysia

A flavonoids-rich extract and seven flavonoids from the leaves and heartwoods of A. anisophyllus Miq. and A. lowii King were investigated for their inhibitory effects against acetylcholinesterase enzyme. The flavonoids were fully characterized spectroscopically as artocarpin (1), isobavachalcone (2), pyranocycloartobiloxanthone A (3), 5,7-dihydroxy-4'-methoxy-6-prenylflavanone (4), 5-hydroxy-7,8-(2,2-dimethylchromano)-4'-methoxy-flavanone (5) 4',5-dihydroxy-6,7-(2,2-dimethylpyrano)-2'-methoxy-8-γ,γ-dimethylallylflavone (6) and 2',4'-dihydroxy-3,4-(2",2"-dimethylchromeno)-3'-prenyldihydrochalcone (7). Acetylcholinesterase inhibitory activity of the samples was determined using TLC plate with bioactivity staining based on the Ellman’s method. Pyranocycloartobiloxanthone A (3) exhibited an excellent inhibitory activity with detection limit at 7.81 µg/mL.