Characterisation and stabilisation of recombinant humicola insolens endoglucanase produced in pichia pastoris

• Main Authors: Abdul Fattah, S. S., Mohamed, Rahmah, Wan Seman, W. M. K., Md. Jahim, Jamaliah, Md. Illias, Rosli, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir
• Format: Article
• Published: Malaysian Society of Applied Biology 2015
• Subjects: Q Science (General)
• Online Access: http://eprints.utm.my/id/eprint/58022/; https://www.cabdirect.org/cabdirect/abstract/20153192850
• Institution: Universiti Teknologi Malaysia

Cellulases are industrially important hydrolytic enzymes that are applicable in the bioconversion of cellulosic biomass to simple sugars. In this work, Pichia pastoris carrying an endoglucanase cDNA (CMC3) from a thermophilic fungus, Humicola insolens, was grown in a 30 L bioreactor to produce recom...