Screening factors influencing the production of astaxanthin from freshwater and marine microalgae

Astaxanthin, a carotenoid pigment found in several aquatic organisms, is responsible for the red colour of salmon, trout and crustaceans. In this study, astaxanthin production from freshwater microalga Chlorella sorokiniana and marine microalga Tetraselmis sp. was investigated. Cell growth and astax...

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Main Authors: Mohamad, Shaza Eva, Jamaluddin, Haryati, Md. Salleh, Madihah, Ibnu Rasid, Elda Nurafnie
Format: Article
Published: Humana Press Inc. 2014
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Online Access:http://eprints.utm.my/id/eprint/62527/
http://dx.doi.org/10.1007/s12010-013-0644-x
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spelling my.utm.625272017-06-18T00:28:34Z http://eprints.utm.my/id/eprint/62527/ Screening factors influencing the production of astaxanthin from freshwater and marine microalgae Mohamad, Shaza Eva Jamaluddin, Haryati Md. Salleh, Madihah Ibnu Rasid, Elda Nurafnie QH Natural history Astaxanthin, a carotenoid pigment found in several aquatic organisms, is responsible for the red colour of salmon, trout and crustaceans. In this study, astaxanthin production from freshwater microalga Chlorella sorokiniana and marine microalga Tetraselmis sp. was investigated. Cell growth and astaxanthin production were determined spectrophotometrically at 620 and 480 nm, respectively. Astaxanthin was extracted using acetone and measured subsequent to biomass removal. Aerated conditions favoured astaxanthin production in C. sorokiniana, whereas Tetraselmis sp. was best cultured under unaerated conditions. C. sorokiniana produced more astaxanthin with the highest yield reached at 7.83 mg/l in 6.0 mM in nitrate containing medium compared to Tetraselmis sp. which recorded the highest yield of only 1.96 mg/l in 1.5 mM nitrate containing medium. Production in C. sorokiniana started at the early exponential phase, indicating that astaxanthin may be a growth-associated product in this microalga. Further optimization of astaxanthin production was performed using C. sorokiniana through a 23 full factorial experimental design, and a yield of 8.39 mg/l was achieved. Overall, the study has shown that both microalgae are capable of producing astaxanthin. Additionally, this research has highlighted C. sorokiniana as a potential astaxanthin producer that could serve as a natural astaxanthin source in the current market. Humana Press Inc. 2014 Article PeerReviewed Mohamad, Shaza Eva and Jamaluddin, Haryati and Md. Salleh, Madihah and Ibnu Rasid, Elda Nurafnie (2014) Screening factors influencing the production of astaxanthin from freshwater and marine microalgae. Applied Biochemistry and Biotechnology, 172 (4). pp. 2160-2174. ISSN 1559-0291 http://dx.doi.org/10.1007/s12010-013-0644-x DOI:10.1007/s12010-013-0644-x
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QH Natural history
spellingShingle QH Natural history
Mohamad, Shaza Eva
Jamaluddin, Haryati
Md. Salleh, Madihah
Ibnu Rasid, Elda Nurafnie
Screening factors influencing the production of astaxanthin from freshwater and marine microalgae
description Astaxanthin, a carotenoid pigment found in several aquatic organisms, is responsible for the red colour of salmon, trout and crustaceans. In this study, astaxanthin production from freshwater microalga Chlorella sorokiniana and marine microalga Tetraselmis sp. was investigated. Cell growth and astaxanthin production were determined spectrophotometrically at 620 and 480 nm, respectively. Astaxanthin was extracted using acetone and measured subsequent to biomass removal. Aerated conditions favoured astaxanthin production in C. sorokiniana, whereas Tetraselmis sp. was best cultured under unaerated conditions. C. sorokiniana produced more astaxanthin with the highest yield reached at 7.83 mg/l in 6.0 mM in nitrate containing medium compared to Tetraselmis sp. which recorded the highest yield of only 1.96 mg/l in 1.5 mM nitrate containing medium. Production in C. sorokiniana started at the early exponential phase, indicating that astaxanthin may be a growth-associated product in this microalga. Further optimization of astaxanthin production was performed using C. sorokiniana through a 23 full factorial experimental design, and a yield of 8.39 mg/l was achieved. Overall, the study has shown that both microalgae are capable of producing astaxanthin. Additionally, this research has highlighted C. sorokiniana as a potential astaxanthin producer that could serve as a natural astaxanthin source in the current market.
format Article
author Mohamad, Shaza Eva
Jamaluddin, Haryati
Md. Salleh, Madihah
Ibnu Rasid, Elda Nurafnie
author_facet Mohamad, Shaza Eva
Jamaluddin, Haryati
Md. Salleh, Madihah
Ibnu Rasid, Elda Nurafnie
author_sort Mohamad, Shaza Eva
title Screening factors influencing the production of astaxanthin from freshwater and marine microalgae
title_short Screening factors influencing the production of astaxanthin from freshwater and marine microalgae
title_full Screening factors influencing the production of astaxanthin from freshwater and marine microalgae
title_fullStr Screening factors influencing the production of astaxanthin from freshwater and marine microalgae
title_full_unstemmed Screening factors influencing the production of astaxanthin from freshwater and marine microalgae
title_sort screening factors influencing the production of astaxanthin from freshwater and marine microalgae
publisher Humana Press Inc.
publishDate 2014
url http://eprints.utm.my/id/eprint/62527/
http://dx.doi.org/10.1007/s12010-013-0644-x
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