A kinetic study of enzymatic hydrolysis of oil palm biomass for fermentable sugar using polyethylene glycol (PEG) immobilized cellulase
In this work, enzymatic hydrolysis by cellulase in a soluble and an immobilized form was studied to convert lignocellulosic oil palm empty fruit bunch (EFB) biomass into fermentable sugars as a feedstock for bioethanol production. The cellulase was covalently immobilized with activated and functiona...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit UTM Press
2016
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/71215/1/IsahNwaha2016_Akineticstudyofenzymatic.pdf http://eprints.utm.my/id/eprint/71215/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988378072&partnerID=40&md5=1ba139417d06940f632efb015120a8b3 |
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| Institution: | Universiti Teknologi Malaysia |
| Language: | English |
| Summary: | In this work, enzymatic hydrolysis by cellulase in a soluble and an immobilized form was studied to convert lignocellulosic oil palm empty fruit bunch (EFB) biomass into fermentable sugars as a feedstock for bioethanol production. The cellulase was covalently immobilized with activated and functionalized polyethylene glycol (PEG) via glutaraldehyde coupling method. As a whole, the immobilized cellulase displayed 50 higher efficiency over free cellulase, in reducing sugar recovery during hydrolysis reactions at pH of 4.8 and temperature of 50°C. From the kinetic study, it showed that Michaelis constant (Km) and limiting velocity (Vmax) of immobilized cellulase were 179.2 mg/ml and 33.5 mg/ml.min respectively, comparable with the value for free cellulose, 171.8 mg/ml and 34.5 mg/ml.min respectively. This result could be attributed to the effect of PEG on the binding cellulase to substrate desorb substrates, and enables free interaction of cellulase to hydrolyse cellulose maximally. |
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