Biotransformation studies of cresol red by Absidia spinosa M15

• Main Authors: Kristanti, R. A., Fikri Ahmad Zubir, M. M., Hadibarata, T.
• Format: Article
• Published: Academic Press 2016
• Subjects: TA Engineering (General). Civil engineering (General)
• Online Access: http://eprints.utm.my/id/eprint/72573/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958967756&doi=10.1016%2fj.jenvman.2015.11.017&partnerID=40&md5=228429242847e732d0fa56baceec5cc7
• Institution: Universiti Teknologi Malaysia

Cresol Red, a commercial dye that used widely to color nylon, wool, cotton, and polyacrylonitrile-modified nylon in the massive textile manufacture is toxic recalcitrant. Absidia spinosa M15, a novel fungal strain isolated from a tropical rain forest, was found to decolorize Cresol Red 65% within 30 d under agitation condition. UV-Vis spectroscopy, TLC analysis and mass spectra of samples after decolorization process in culture medium confirmed final decolorization of Cresol Red. Two metabolites were identified in the treated medium: benzeneacetic acid (tR 9.6 min and m/z 136) and benzoic acid (tR 5.7 min and m/z 122). Laccase showed the significant activity (133.8 U/L) in biomass obtained at the end of experiment demonstrates role of the enzyme in the decolorization process.